Experiments in Plant Tissue Culture (Hardcover, 3rd)

Part I. Background Information: 1. Culture of plant cells, tissues, and organs; 2. Laboratory facilities; 3. Aseptic techniques; 4. Media composition and preparation; Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus; 6. Organogenesis; 7. Cell suspensions; 8. Somatic embryogenesis; Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots; 10. Micropropagation by bud proliferation; 11. Anther and pollen cultures; Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements; 13. Isolation and culture of protoplasts; 14. Protoplast fusion and somatic hybridization; Part V. Supplementary Topics: 15. Cryopreservation of germplasm; 16. Production of secondary metabolites; 17. Quantitation of procedures; 18. Formulations of tissue culture media; 19. Commercial sources of supplies; Author index; Subject index.