Transcription Factor Protocols (Hardcover, 2000)

Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method Dennis K. Watson, Richard Kitching, Calvin Vary, Ismail Kola, and Arun Seth In Vivo Footprinting Using UV Light and Ligation-Mediated PCR Gerd P. Pfeifer and Stella Tommasi Identification of DNaseI Hypersensitive Sites Within Nuclei Peter N. Cockerill Analysis of In Vivo Methylation Hans-Henrik M. Dahl and Wendy M. Hutchison Detection of Transcription Factor Partners with a Yeast One Hybrid Screen Michael Sieweke Inverse PCR (IPCR) for Obtaining Promoter Sequence Tony Triglia PCR-Directed Linker Scanning Mutagenesis Kurt Gustin and Robert D. Burk Transfection Technologies Elaine T. Schenborn The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues Charles M. Nicolet and Ning-Sun Yang Optimizing Electroporation Conditions for the Transformation of Mammalian Cells William C. Heiser Calcium Phosphate Transfection of Mammalian Cultured Cells Elaine T. Schenborn and Virginia Goiffon DEAE-Dextran Transfection of Mammalian Cultured Cells Elaine T. Schenborn and Virginia Goiffon Liposome-Mediated Transfection of Mammalian Cells Elaine T. Schenborn and Jennifer Oler Assays for Transcriptional Activity Based on the Luciferase Reporter Gene S. Roy Himes and M. Frances Shannon Transient Transfection of Schneider Cells in the Study of Transcription Factors Guntram Suske Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription Marina Kochetkova and M. Frances Shannon Expression and Purification of Histidine-Tagged Transcription Factors Donna R. Cohen Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein Ulrich Kruse, Thorsten T. Ebel, and Albrecht E. Sippel Electrophoretic Mobility Shift Assays Peter L. Molloy In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors Martin J. Tymms In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors Fujiko Watt Computer Software of Eukaryotic Promoter Analysis Dan S. Prestridge Index