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Structural Proteomics: High-Throughput Methods

Structural Proteomics: High-Throughput Methods (Paperback)

J. Raymond (엮은이)
Humana
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Structural Proteomics: High-Throughput Methods
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· 제목 : Structural Proteomics: High-Throughput Methods (Paperback) 
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 생화학
· ISBN : 9781071614082
· 쪽수 : 344쪽
· 출판일 : 2022-05-07

목차

Part I: Structural Bioinformatics

 

1. The Protein Data Bank Archive

            Sameer Velankar, Stephen K. Burley, Genji Kurisu, Jeffery C. Hoch, and John L. Markley

 

2. Computational Methods for the Elucidation of Protein Structure and Interactions

            Nicholas S. Edmunds and Liam J. McGuffin

 

3. Methods for Molecular Modeling of Protein Complexes

            Tejashree Rajaram Kanitkar,, Neeladri Sen, Sanjana Nair, Neelesh Soni, Kaustubh Amritkar, Yogendra Ramtirtha, and M.S. Madhusudhan

 

Part II: Protein Production

 

4. High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus

            Ester Behiels and Jonathan Elegheert

 

5. Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies

            Ganna O. Krasnoselska, Maud Dumoux, Nadisha Gamage, Harish Cheruvara, James Birch, Andrew Quigley, and Raymond J. Owens

 

6. Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

            Maren Schubert, Manfred Nimtz, Federico Bertoglio, Stefan Schmelz, Peer Lukat, and Joop van den Heuvel

 

7. SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering

            Hannah Crocker, Barbara Gorda, Martin Pelosse, Deepak Balaji Thimiri Govinda Raj, and Imre Berger

 

8. Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification

            Sylvain Geny, Simon Pichard, Arnaud Poterszman, and Jean-Paul Concordet

 

9. Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems

            Marlitt Stech, Nathanael Rakotoarinoro, Tamara Teichmann, Anne Zemella, Lena Thoring, and Stefan Kubick

 

Part III: Structure Determination

 

10. Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

            Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, Jijumon A.S., Carsten Janke3, Anna Akhmanova, and Marc Baldus

 

11. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources

            Pierre Aller and Allen M. Orville

 

12. From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example

            Christoph A. Diebolder, Rebecca S. Dillard, and Ludovic Renault

 

13. Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods

            Carlos Oscar S. Sorzano, Amaya Jimenez-Moreno, David Maluenda, Erney Ramirez-Aportela, Marta Martinez, Ana Cuervo, Robert Melero, Jose Javier Conesa, Ruben Sanchez-Garcia, David Strelak, Jiri Filipovic, Estrella Fernandez-Gimenez, Federico de Isidro-Gomez, David Herreros, Pablo Conesa, Laura del Cano, Yunior Fonseca, Jorge Jimenez de la Morena, Jose Ramon Macias, Patricia Losana, Roberto Marabini, and Jose-Maria Carazo

 

14. Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection

            Ottilie von Loeffelholz and Bruno P. Klaholz

 

15. Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology

            Jana Moravcova, Radka Dopitova, Matya? Pinkas, and Ji?i Nova?ek

 

16. Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED

            Emma Danelius and Tamir Gonen

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