책 이미지
책 정보
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명공학
· ISBN : 9781071619971
· 쪽수 : 435쪽
· 출판일 : 2022-01-06
목차
Part I: Tools for Cell-Free Expression Systems
1. Best Practices for DNA Template Preparation towards Improved Reproducibility in Cell-Free Protein Production
Eugenia F. Romantseva, Drew S. Tack, Nina Alperovich, David Ross, and Elizabeth A. Strychalski
2. Simple Extract Preparation Methods for E. coli-Based Cell-Free Expression
Alissa C. Mullin, Taylor Slouka, and Javin P. Oza
3. Preparation and Screening of Cell-Free Extract from Non-Growing Escherichia coli A19 Cells
Florian Hiering, Jurek Failmezger, and Martin Siemann-Herzberg
4. Cell-Free Protein Synthesis Using Pichia pastoris
Alex J. Spice, Rochelle Aw, and Karen M. Polizzi
5. A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression
Huiling Xu, Wan-Qiu Liu, and Jian Li
6. In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins
Hossein Moghimianavval, Yen-Yu Hsu, Alessandro Groaz, and Allen P. Liu
7. High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems
Conary Meyer, Chuqing Zhou, Zecong Fang, Marjorie L. Longo, Tingrui Pan, and Cheemeng Tan
8. Cell-Free Gene Expression from DNA Brushes
Michael Levy, Ohad Vonshak, Yiftach Divon, Ferdinand Greiss, Noa Avidan, Shirley S. Daube, and Roy H. Bar-Ziv
9. Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications
Kazuaki Amikura, Keita Hibi, and Yoshihiro Shimizu
10. Measurement of Transcription, Translation, and Other Enzymatic Processes during Cell-Free Expression Using PERSIA
Scott Wick and Peter A. Carr
Part II: Applications of Cell-Free Expression Systems
11. Cell-Free Noncanonical Redox Cofactor Systems
William B. Black and Han Li
12. Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping
Blake J. Rasor, Bastian Vogeli, Michael C. Jewett, and Ashty S. Karim
13. Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry
April M. Miguez, Yan Zhang, and Mark P. Styczynski
14. Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus
Orion M. Venero, Wakana Sato, Joseph M. Heili, Christopher Deich, and Katarzyna P. Adamala
15. Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression
Imre Banlaki, Francois-Xavier Lehr, and Henrike Niederholtmeyer
16. Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles
Miranda L. Jacobs and Neha P. Kamat
17. Cell-Free Synthesis Strategies to Probe Co-Translational Folding of Proteins within Lipid Membranes
Nicola J. Harris, Eamonn Reading, and Paula J. Booth
18. Assembly of RNA Nanostructures from Double Crossover Tiles
Jaimie Marie Stewart, Hari K.K. Subramanian, and Elisa Franco
19. Cell-Free Biosensors and AI Integration
Paul Soudier, Leon Faure, Maish Kushwaha, and Jean-Loup Faulon
20. ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors
Jaeyoung K. Jung, Khalid K. Alam, and Julius B. Lucks
21. Optical Sensing in Cell-Free Expression
Junzhu Yang and Yuan Lu
22. Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers
Melissa K. Takahashi, Xiao Tan, and Aaron J. Dy
23. Detection of Norovirus Using Paper-Based Cell-Free Systems
Kaiyue Wu and Alexander A. Green
24. A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes
Franziska Wimmer, Frank Englert, and Chase L. Beisel
25. Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology
Ally Huang, Bruce Bryan, Sebastian Kraves, Ezequiel Alvarez-Saavedra, and Jessica C. Stark
