Hdac/Hat Function Assessment and Inhibitor Development: Methods and Protocols (Hardcover, 2, Second 2023)

· 제목 : Hdac/Hat Function Assessment and Inhibitor Development: Methods and Protocols (Hardcover, 2, Second 2023) 
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 생화학
· ISBN : 9781071627877
· 쪽수 : 511쪽
· 출판일 : 2022-10-19

Part I: In Vivo and 3R Models to Assess HDAC Functions and Effects of HDACi

 

1. Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models

            Eva-Maria Piskor, Rene Winkler, and Christian Kosan

 

2. Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells

            Miriam Pons and Mandy Beyer

 

3. Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes

            Roland Windisch, Sophie Kreissig, and Christian Wichmann

 

4. Development of a Cellular Model Mimicking Specific HDAC Inhibitors

            Lena Hess, Verena Moos, and Christian Seiser

 

5. Evaluation of Anti-Tumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model

            Charlotte Gatzweiler, Johannes Ridinger, Simay Ayhan, Sara Najafi, Heike Peterziel, Olaf Witt, and Ina Oehme

 

6. Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation

            Miriam Pons, Roland Conradi, Walburgis Brenner, and Oliver H. Kramer

 

7. Investigating Physiopathological Roles for Sirtuins in a Mouse Model

            Shimin Sun, Xiaojiao Xia, Ming Wang, and Baohua Liu

 

8. Patient-Derived Organoids for In Vivo Validation of In Vitro Data

            Said Kdimati, Florian Burtin, Michael Linnebacher, and Christina Susanne Mullins

 

Part II: Novel HDAC Inhibitors and Improved Formulations for HDAC Inhibitors

 

9. Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges

            Marie Kuhne, Susanne Hofmann, Henry Lindemann, Zoltan Cseresnyes, Andreas Dzierza, Daniel Schroder, Maren Godmann, Andreas Koschella, Christian Eggeling, Dagmar Fischer, Marc Thilo Figge, Thomas Heinze, and Thorsten Heinzel

 

10. Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1-3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines

            Mohamed Abdelsalam, Hany S. Ibrahim, Lukas Krauss, Matthes Zessin, Anita Vecchio, Sieglinde Hastreiter, Mike Schutkowski, Gunter Schneider, and Wolfgang Sippl

 

11. Evaluation of Small Molecule HDAC Inhibitors through In Vitro and In Cellulo Approaches

            Nabanita Nawar, Harsimran Kaur Garcha, Abootaleb Sedighi, Elvin D. de Araujo, and Patrick T. Gunning

 

12. Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras

            Salma Darwish, Tino Heimburg, Johannes Ridinger, Daniel Herp, Matthias Schmidt, Christophe Romier, Manfred Jung, Ina Oehme, and Wolfgang Sippl

 

13. HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles

            Henry Lindemann, Marie Kuhne, Andreas Koschella, Maren Godmann, Thorsten Heinzel, and Thomas Heinze

 

14. Synthesis and Characterization of Reversible Covalent HDAC4 Inhibitors

            Anton Fruhauf, Benjamin Wolff, Markus Schweipert, and Franz-Josef Meyer-Almes

 

Part III: Innovative Techniques to Determine the Impact of Acetylation on Chromatin, DNA Damage, and Cell Stress Responses

 

15. Analysis of Cell Cycle and DNA Compaction Dependent Subnuclear Distribution of Histone Marks

            Sunil Kumar Pradhan and M. Cristina Cardoso

 

16. Analyzing the Effects of HDAC Inhibitors on DNA Damage and Associated Cytotoxicity in Primary Hepatocytes

            Max J. Carlsson and Jorg Fahrer

 

17. Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) Stress

            Melisa Halilovic, Lisa Marx-Blumel, Christian Marx, Katrin Buder, James F. Beck, and Jurgen Sonnemann

 

18. Assessment of Mitochondrial Dysfunctions after Sirtuin Inhibition

            Christian Marx, Lisa Marx-Blumel, Jurgen Sonnemann, and Zhao-Qi Wang

 

19. Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Non-Homologous End Joining

            Oliver H. Kramer, Tamara Diehl, and Wynand P. Roos

 

20. Assessing SIRT7 Activity In Vivo and In Vitro in Response to DNA Damage

            Arif Ali Chishti, Zhenhai Li, Baohua Liu, and Wei-Guo Zhu

 

21. Methods to Evaluate the Effects of HAT/KAT Inhibition on SIAH2-Driven Reactive Oxygen Species Generation in Helicobacter pylori-Infected Gastric Epithelial Cells

            Pragyesh Dixit, Shrikant Babanrao Kokate, Suvasmita Rath, Lopamudra Das, Debashish Chakraborty, and Asima Bhattacharyya

 

22. Monitoring Changes in Intracellular Reactive Oxygen Species Levels in Response to Histone Deacetylase Inhibitors

            Al-Hassan M. Mustafa, Ramy Ashry, and Oliver H. Kramer

 

23. Single-Cell Analysis of Histone Acetylation Dynamics at Replication Forks Using PLA and SIRF

            Seo Yun Lee, Jae Jin Kim, and Kyle M. Miller

 

24. Testing the Effect of Histone Acetyltransferases on Local Chromatin Compaction

            Maximilian Pfisterer and M. Lienhard Schmitz

 

Part IV: Modern Assays to Evaluate HDAC Functions and HDACi, Including CRISPR-Cas9 Techniques

 

25. A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virotherapy

            Michela Muscolini, John Hiscott, and Evelyne Tassone

 

26. Cloning Strategy for HDAC1/HDAC2 Hybrid Protein Expression in Mammalian Cells

            Desiree Gul, Sandra Olf, Jan Hagemann, Roland H. Stauber, and Oliver H. Kramer

 

27. Continuous Histone Deacylase Activity Assays

            Matthes Zessin, Marat Meleshin, Wolfgang Sippl, and Mike Schutkowski

 

28. CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor Resistant Cells

            Paul Jung, Laura Schmalbrock, and Matthias Wirth

 

29. Determining Potency of Inhibitors Targeting Histone Deacetylase 6 by Quantification of Acetylated Tubulin in Cells

            Jana Mikesova, Marketa Ondrakova, Iva Jelinkova, Jakub Ptacek, Zora Novakova, and Cyril Barinka

 

30. Expression and Crystallization of HDAC6 Tandem Catalytic Domains

            Gerasimos Langousis, Jacint Sanchez, Georg Kempf, and Patrick Matthias

 

31. Fluorescence Polarization-Based Competition Assays to Evaluate Histone Deacetylase 6 Inhibitors

            Qirat F. Ashraf, Erica J. Quilates, Olasunkanmi O. Olaoye, Elvin D. de Araujo, and Patrick T. Gunning

 

32. Immunoprecipitation of HDAC6 and Interacting Proteins

            Longlong Wang, Jacint Sanchez, Daniel Hess, and Patrick Matthias