[eBook Code] Biological Field Emission Scanning Electron Microscopy (eBook Code, 1st)

About the Editors xix

List of Contributors xxi

Foreword xxv

1 Scanning Electron Microscopy: Theory, History and Development of the Field Emission Scanning Electron Microscope 1
David C. Joy

2 Akashi Seisakusho Ltd – SEM Development 1972–1986 7
Michael F. Hayles

3 Development of FE-SEM Technologies for Life Science Fields 25
Mitsugu Sato, Mami Konomi, Ryuichiro Tamochi and Takeshi Ishikawa

4 A History of JEOL Field Emission Scanning Electron Microscopes with Reference to Biological Applications 53
Kazumichi Ogura and Andrew Yarwood

5 TESCAN Approaches to Biological Field Emission Scanning Electron Microscopy 79
Jaroslav Jiruše, Vratislav Košˇtál and Bohumila Lencová

6 FEG-SEM for Large Volume 3D Structural Analysis in Life Sciences 103
Ben Lich, Faysal Boughorbel, Pavel Potocek and Emine Korkmaz

7 ZEISS Scanning Electron Microscopes for Biological Applications 117
Isabel Angert, Christian Böker, Martin Edelman, Stephan Hiller, Arno Merkle and Dirk Zeitler

8 SEM Cryo-Stages and Preparation Chambers 143
Robert Morrison

9 Cryo–SEM Specimen Preparation Workflows from the Leica Microsystems Design Perspective 167
Guenter P. Resch

10 Chemical Fixation 191
Bruno M. Humbel, Heinz Schwarz, Erin M. Tranfield and Roland A. Fleck

11 A Brief Review of Cryobiology with Reference to Cryo Field Emission Scanning Electron Microscopy 223
Roland A. Fleck, Eyal Shimoni and Bruno M. Humbel

12 High-Resolution Cryo-Scanning Electron Microscopy of Macromolecular Complexes 265
Sebastian Tacke, Falk Lucas, Jeremy D. Woodward, Heinz Gross and Roger Wepf

13 FESEM in the Examination of Mammalian Cells and Tissues 299
Andrew Forge, Anwen Bullen and Ruth Taylor

14 Public Health/Pharmaceutical Research – Pathology and Infectious Disease 311
Paul A. Gunning and Bärbel Hauröder

15 Field Emission Scanning Electron Microscopy in Cell Biology Featuring the Plant Cell Wall and Nuclear Envelope 343
Martin W. Goldberg

16 Low-Voltage Scanning Electron Microscopy in Yeast Cells 363
Masako Osumi

17 Field Emission Scanning Electron Microscopy in Food Research 385
Johan Hazekamp and Marjolein van Ruijven

18 Cryo-FEGSEM in Biology 397
Paul Walther

19 Preparation of Vitrified Cells for TEM by Cryo-FIB Microscopy 415
Yoshiyuki Fukuda, Andrew Leis and Alexander Rigort

20 Environmental Scanning Electron Microscopy 439
Rudolph Reimer, Dennis Eggert and Heinrich Hohenberg

21 Correlative Array Tomography 461
Thomas Templier and Richard H.R. Hahnloser

22 The Automatic Tape Collection UltraMicrotome (ATUM) 485
Anwen Bullen

23 SBEM Techniques 495
Christel Genoud

24 FIB-SEM for Biomaterials 517
Lucille A. Giannuzzi

25 New Opportunities for FIB/SEM EDX in Nanomedicine: Cancerogenesis Research 533
Damjana Drobne, Sara Novak, Andreja Erman and Goran Dra?i´c

26 FIB-SEM Tomography of Biological Samples: Explore the Life in 3D 545
Caroline Kizilyaprak, Damien De Bellis, Willy Blanchard, Jean Daraspe and Bruno M. Humbel

27 Three-Dimensional Field-Emission Scanning Electron Microscopy as a Tool for Structural Biology 567
J.D. Woodward and R.A. Wepf

28 Element Analysis in the FEGSEM: Application and Limitations for Biological Systems 589
Alice Warley and Jeremy N. Skepper

29 Image and Resource Management in Microscopy in the Digital Age 611
Patrick Schwarb, Anwen Bullen, Dean Flanders, Maria Marosvölgyi, Martyn Winn, Urs Gomez and Roland A. Fleck

30 Part 1: Optimizing the Image Output: Tuning the SEM Parameters for the Best Photographic Results 625
Oliver Meckes and Nicole Ottawa

31 A Synoptic View on Microstructure: Multi-Detector Colour Imaging, nanoflight^® 659
Stefan Diller

Index 679