Laboratory Protocols in Fungal Biology: Current Methods in Fungal Biology (Hardcover, 2013)

1. Safety Norms and Regulations in Handling Fungal Specimens Finola E. Cliffe 2. Methods of Cryopreservation in Fungi Ladislav Homolka 3. Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results Svetlana M. Ozerskaya, Natalya E. Ivanushkina, Galina A. Kochkina, Svetlana S. Eremina, Alexander N. Vasilenko, and Nadezhda I. Chigineva 4. Fungal Specimen Collection and Processing. Anthonia O'Donovan, Vijai Kumar Gupta, and Maria G. Tuohy 5. Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops S. Chandra Nayaka, A.C. Udayashankar, M.Venkata Ramana, S.R. Niranjana, C. N. Mortensen, and H.S. Prakash 6. Identification Key for the Major Growth Forms of Lichenized Fungi Jayabalan Sangeetha and Devarajan Thangadurai 7. Microscopic Methods for Analytical Studies of Fungi De-Wei Li 8. Scanning Electron Microscopy for Fungal Sample Examination Eduardo Alves, Gilvaine Ciavareli Lucas, Edson Ampelio Pozza, and Marcelo de Carvalho Alves 9. High Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy Tanya Dahms 10. Use of Fourier-Transform Infrared (FTIR) Microscopy Method For Detection of Phyto-Fungal Pathogens Vitaly Erukhimovitch and Mahmoud Huleihel 11. Diagnose of Parasitic Fungi in The Plankton: Technique for Identifying And Counting Infective Chytrids Using Epifluorescence Microscopy Telesphore Sime-Ngando, Serena Rasconi, and Melanie Gerphagnon 12. Fungal Cell Wall Analysis Pilar Perez and Juan C. Ribas 13. Histopathological Technique for Detection of Fungal Infections in Plants Vijai Kumar Gupta and Brejesh Kumar Pandey 14. Development of Media for Growth and Enumeration of Fungi from Water Segula Masaphy 15. Sabouraud Agar for Fungal Growth Janelle M. Hare 16. A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and its Application to Antifungal Susceptibility Testing Christopher G. Pierce, Priya Uppuluri, and Jose L. Lopez-Ribot 17. Screening for Compounds Exerting Antifungal Activities Jean-Paul Ouedraogo, Ellen L. Lagendijk, Cees A.M.J.J van den Hondel, Arthur F.J. Ram, and Vera Meyer 18. Fluorescence In situ Hybridization of Uncultured Zoosporic Fungi Telesphore Sime-Ngando, Marlene Jobard, and Serena Rasconi 19. Staining Techniques and Biochemical Methods for the Identification of Fungi Jayabalan Sangeetha and Devarajan Thangadurai 20. Protocol for the In vivo Quantification of Superoxide Radical in Fungi Grintzalis Konstantinos, Papapostolou Ioannis, and Christos Georgiou 21. Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis Jeannette Vogt, Frank Stahl, Thomas Scheper, and Susann Muller 22. Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi Jose Javiar de Vega-Bartol, Maria Vega Tello Hernandez, Jonathan Nino Sanchez, Virginia Casado del Castillo, and Jose M. Diaz-Minguez 23. Semi-nested PCR Approach to Amplify Large 8S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities Miruna Oros-Sichler and Kornelia Smalla 24. Proteomic Protocols for the Study of Filamentous Fungi Raquel Gonzalez Fernandez and Jesus V. Jorrin Novo 25. Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay Anthony J. O'Donoghue and Cathal S. Mahon 26. Protocol of a LightCyclerO PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients Birgit Spiess and Dieter Buchheidt 27. Application of Polymerase Chain Reaction (PCR) and PCR Based Methods Targeting Internal Transcribed Spacer (ITS) for Detection and Species Level Identification of Fungi K. Lily Therese, R. Bagyalakshmi, and H. N. Madhavan 28. Real-Time PCR Assay in Fungi