Protein-Protein Interactions: Methods and Applications (Paperback, 2, Softcover Repri)

PART I OVERVIEWS 1 Structural basis of protein-protein interactions Robert Liddington 2 Quantitative analysis of protein-protein interactions Ziad Eletr and Keith Wilkinson 3 Protein-protein interaction databases Damian Szklarczyk and Lars Juhl Jensen 4 Computational prediction of protein-protein interactions Tobias Ehrenberger, Lewis C. Cantley, and Michael B. Yaffe 5 Structure-based computational approaches for small-molecule modulation of protein-protein interactions David Xu, Bo Wang, and Samy O. Meroueh 6 Targeting protein-protein interactions for drug discovery David C. Fry PART II LABEL-FREE BIOSENSORS AND TECHNIQUES 7 Studying protein-protein interactions using surface plasmon resonance Zenata Nikolovska-Coleska 8 Resonant waveguide grating for monitoring bimolecular interactions Meng Wu and Min Li 9 Quartz microbalance technology for probing biomolecular interactions Gabriella T. Heller, Alison R. Mercer-Smith, and Malkiat S. Johal 10 Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry Sriram Kumaraswamy and Renee Tobias 11 Characterization of protein-protein interactions by isothermal titration calorimetry Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freiere 12 Sedimentation equilibrium studies Ian A. Taylor, John F. Eccleston, and Katrin Rittinger 13 Detecting protein-protein interactions by gel filtration chromatography Yan Bai 14 Using light scattering to determine the stoichiometry of protein complexes Jeremy Mogridge 15 Circular dichroism (CD) analyses of protein-protein interactions Norma Greenfield 16 Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy Peter M. Thompson, Moriah R. Beck, and Sharon L. Campbell 17 Quantitative protein analysis by mass spectrometry Vishwajeeth Pagala, Anthony High , Xusheng Wang, Haiyan Tan, Kiran Kodali, Ashutosh Mishra , Kanisha Kavdia , Yanji Xu, Zhiping Wu, and Junmin Peng 18 Using peptide arrays created by the SPOT method for defining protein-protein interactions Yun Young Yim, Katherine Betke, and Heidi Hamm PART III TAG/AFFINITY-BASED METHODS 19 Fluorescence polarization assay to quantify protein-protein interactions Ronald T. Raines 20 Forster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions Alexa L. Mattheyses and Adam I. Marcus 21 Utilizing ELISA to monitor protein-protein interactions Zusen Weng and Qinjian Zhao 22 Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions Haris G. Vikis and Kun-Liang Guan 23 Hexahistidine (6xHis) fusion-based assays for protein-protein interactions Mary C. Puckett 24 Studying protein-protein interactions via blot overlay/Far Western blot Randy A. Hall 25 Co-immunoprecipitation from transfected cells Yoshinori Takahashi 26 In vivo protein cross-linking Fabrice Agou and Michel V eron PART IV CELL-BASED BIMOLECULAR INTERACTION REPORTER ASSAYS 27 Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening Jeroen Wagemans and Rob Lavigne 28 Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn 29 MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions Irma Lemmens, Sam Lievens, and Jan Tavernier 30 Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells Nicole E. Brown, Joe B. Blumer, and John R. Hepler 31 Mapping biochemical networks with protein fragment complementation assays Ingrid Remy and Stephen W. Michnick 32 Detection of protein-protein interaction using bimolecular fluorescence complementation assay Cau D. Pham 33 Split-luciferase complementation assay to detect channel-protein interactions in liv