Guanylate Cyclase and Cyclic GMP: Methods and Protocols (Paperback, Softcover Repri)

1.NO-cGMP: The Past, the Present and the Future Michael Russwurm, Corina Russwurm, Doris Koesling, and Evanthia Mergia 2. cGMP Dependent Protein Kinases (cGK)Franz Hofmann and Jorg W. Wegener 3. Enzyme Assays for cGMP Hydrolysing Phosphodiesterases Sergei D. Rybalkin, Thomas R. Hinds and Joseph A. Beavo 4. Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues Ronald Jager, Dieter Groneberg, Barbara Lies, Noomen Bettaga, Michaela Kummel, and Andreas Friebe 5. Hyperspectral Imaging of FRET-based cGMP Probes Thomas C. Rich, Andrea L. Britain, Tiffany Stedman, and Silas J. Leavesley 6. Visualization of cGMP with cGi Biosensors Martin Thunemann, Natalie Fomin, Christian Krawutschke, Michael Russwurm, and Robert Feil 7. Advances and techniques to measure cGMP in intact cardiomyocytesKonrad R. Gotz and Viacheslav O. Nikolaev 8. Real-time Monitoring the Spatio-temporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle CellsKara F. Held and Wolfgang R. Dostmann 9. Methods for Identification of cGKI Substrates Katja Salb and Jens Schlossmann 10. Approaches for Monitoring PKG1a Oxidative Activation Joeseph R. Burgoyne and Philip Eaton 11. Analysis of cGMP Signaling in Adipocytes Katja Jennissen, Bodo Haas, Michaela M. Mitschke, Franziska Siegel and Alexander Pfeifer 12. A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signalling Mutant Mice Hannes Schmidt, Gohar Ter-Avetisyan and Fritz G. Rathjen 13. Receptor Binding Assay for NO-Independent Activators of soluble Guanylate Cyclase Peter M. Schmidt and Johannes-Peter Stasch 14. Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing Ruirui Lu and Achim Schmidtko 15. The Geisler Method: Tracing Activity Dependent cGMP Plasticity Changes Upon Double Detection of mRNA and Protein on Brain Slices Wibke Singer, Hyun-Soon Geisler and Marlies Knipper 16. Detection of cGMP in the Degenerating Retina Stylianos Michalakis, Jianhua Xu and Martin Biel, Xi-Qin Ding