Tau Protein: Methods and Protocols (Hardcover, 2017)

'Preface Table of Contents Contributors Part I Native Tau Protein: Conformational Studies and Tubulin Interactions 1. Conformational Dynamics of Intracellular Tau Protein Revealed by CD and SAXS Nalini Vijay Gorantla, Alexander V. Shkumatov, and Subashchandrabose Chinnathambi 2. Global Conformation of Tau Protein Mapped by Raman Spectroscopy Nalini Vijay Gorantla, Puneet Khandelwal, Pankaj Poddar, and Subashchandrabose Chinnathambi 3. Molecular Dynamics Simulation of Tau Peptides for the Investigation of Conformational Changes Induced by Specific Phosphorylation Patterns Neha S.Gandhi, Predrag Kukic, Guy Lippens, and Ricardo L. Mancera 4. Tau Interaction with Tubulin and Microtubules: From Purified Proteins to Cells Tiphany De Bessa, Gilles Breuzard, Diane Allegro, Francois Devred, Vincent Peyrot, and Pascale Barbier Part II In Vitro Self-Association of Tau Protein: Oligomer and Fibril Formation 5. X-Ray Structural Study of Amyloid-Like Fibrils of Tau Peptides Bound to Small-Molecule Ligands Einav Tayeb-Fligelman and Meytal Landau 6. Detection and Quantification Methods for Fibrillar Products of In Vitro Tau Aggregation Assays Niki Nanavaty, Lauren Lin, Samantha H. Hinckley, and Jeff Kuret 7. Fourier Transform Infrared (FTIR) Spectroscopy, Ultraviolet Resonance Raman (UVRR) Spectroscopy and Atomic Force Microscopy (AFM) for Study of the Kinetics of Formation and Structural Characterization of Tau Fibrils Gayathri Ramachandran 8. Assays for the Screening and Characterization of Tau Aggregation Inhibitors Janet E. Rickard, David Horsley, Claude M. Wischik, and Charles R. Harrington 9. Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo Julia E. Gerson, Urmi Sengupta, and Rakez Kayed < Part III Tau Post-Translational Modifications 10. Mass Spectrometry Analysis of Lysine Post-Translational Modifications of Tau Protein from Alzheimer's Disease Brain Stefani N. Thomas and Austin J. Yang 11. The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein Haoling Qi, Clement Despres, Sudhakaran Prabakaran, Francois-Xavier Cantrelle, Beatrice Chambraud, Jeremy Gunawardena, Guy Lippens, Caroline Smet-Nocca, and Isabelle Landrieu 12. Tag-Free Semi-Synthesis of Tau Protein Oliver Reimann, Caroline Smet-Nocca, and Christian P. R. Hackenberger 13. Production of O-GlcNAc Modified Recombinant Tau in E.coli and Detection of Ser400 O-GlcNAc Tau In Vivo Scott A. Yuzwa and David J. Vocadlo Part IV Detecting Tau Proteins, Their Modifications, Mutations and Interacting Partners 14. Two-Dimensional Electrophoresis Protocols to Analyze the Microtubule-Associated Tau Proteins from Several Biological Sources Nicolas Sergeant, Francisco-Jose Fernandez-Gomez, Helene Obriot, Sabiha Eddarkaoui, Valerie Buee-Scherrer, and Luc Buee 15. A Simple Method to Avoid Non-Specific Signal When Using Monoclonal Anti-Tau Antibodies in Western Blotting of Mouse Brain Proteins Franck R. Petry, Samantha B. Nicholls, Sebastien S. Hebert, and Emmanuel Planel 16. Flow Cytometry Analysis and Quantitative Characterization of Tau in Synaptosomes from Alzheimer's Disease Brains Karen Hoppens Gylys and Tina Bilousova 17. In Vivo Microdialysis of Brain Interstitial Fluid for the Determination of Extracellular Tau Levels Kaoru Yamada 18. Proximity Ligation Assay: A Tool to Study Endogenous Interactions between Tau and its Neuronal Partners Alexis Bretteville, Florie Demiautte, and Julien Chapuis 19. Finding MAPT Mutations in Frontotemporal Dementia and Other Tauopathies Carol Dobson-Stone and John B. J. Kwok Part V Cellular and In Vivo Models of Tau Physiopathology 20. Tracking Tau in Neurons: How to Grow, Fix and Stain Primary Neuron