Protein Engineering: Methods and Protocols (Hardcover, 2018)

1. Protein Engineering - Past, Present, and Future Stefan Lutz and Samantha M. Iamurri Part I 2. (Semi)rational Protein Design Ivan V. Korendovych 3. Computational Analysis of Protein Tunnels and Channels Jan Brezovsky, Barbora Kozlikova, and Jiri Damborsky 4. YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations Henrik Land and Maria Svedendahl Humble 5. A Computational Library Design Protocol for Rapid Improvement of Protein Stability - FRESCO Hein J. Wijma, Maximilian J.L.J. Furst, and Dick B. Janssen 6. Directed Evolution of Proteins Based on Mutational Scanning Carlos G. Acevedo-Rocha, Matteo Ferla, and Manfred T. Reetz Part II 7. A Brief Guide to the High-throughput Expression of Directed Evolution Libraries Ana Luisa Ribeiro, Mario Mencia, and Aurelio Hidalgo 8. Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli using MTP Shakers Sandy Schmidt, Mark Dorr, and Uwe T. Bornscheuer 9. Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification Javier Santos-Aberturas, Mark Dorr, and Uwe T. Bornscheuer 10. Functional Analysis of Membrane Proteins Produced by Cell-Free Translation Srujan Kumar Dondapati, Doreen A Wustenhagen, and Stefan Kubick PART III 11. Practical Considerations Regarding the Choice of the Best High-throughput Assay Carolin Mugge and Robert Kouristb 12. High-throughput Screening Assays for Lipolytic Enzymes Alexander Fulton, Marc R. Hayes, Ulrich Schwaneberg, Jorg Pietruszka, and Karl-Erich Jaeger 13. Continuous High-throughput Colorimetric Assays for -transaminases Egon Heuson, Jean-Louis Petit, Franck Charmantray, Veronique de Berardinis, and Thierry Gefflaut 14. Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases Isabel Pardo and Susana Camarero 15. Directed Co-evolution of Two Cellulosic Enzymes in Escherichia coli Based on their Synergistic Reactions Min Liu, Lidan Ye, and Hongwei Yu 16. Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation Mark Dorr and Uwe T. Bornscheuer 17. Solid Phase Agar Plate Assay for Screening Amine Transaminases Martin S. Weiß, Uwe T. Bornscheuer, and Matthias Hohne 18. Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics Fabrice Gielen, Pierre-Yves Colin, Philip Mair, and Florian Hollfelder 19. Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display Christian Schroter, Simon Krah, Jan Beck, Doreen Konning, Julius Grzeschik, Bernhard Valldorf, Stefan Zielonka, and Harald Kolmar 20. Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus Jorg Claren, Thomas Schwab, and Reinhard Sterner