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· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명공학
· ISBN : 9781493984619
· 쪽수 : 397쪽
· 출판일 : 2018-08-30
목차
Part I: Reagents and Kits for Measuring Cells and their Phenotypes
1. Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays
Roy Edward
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2. General Staining and Segmentation Procedures for High Content Imaging and Analysis
Kevin M Chambers, Bhaskar S Mandavilli, Nick J Dolman and Michael S Janes
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3. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis?Bhaskar S Mandavilli, Robert J Aggeler, and Kevin Chambers
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4. Cell-based High Content Analysis of Cell Proliferation and Apoptosis
Bhaskar S Mandavilli, Michelle Yann and Scott Clarke
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5. Tools to Measure Autophagy using High Content Imaging and Analysis
Nick J Dolman, Brent A. Samson, Kevin M. Chambers, Michael S Janes, and Bhaskar S Mandavilli?
Part II: Selecting Microtiter Plates, Identify Imaging Artifacts, Rnai Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS
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6. Guidelines for Microplate Selection in High Content Imaging
O.J. Trask
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7. Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler
Mark-Anthony Bray and Anne E. Carpenter
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8. High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening
Steven A. Haney?
9. Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays
Karol Kozak, Bernt Rinn, Oliver Leven, and M. Emmenlauer
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10. Live-Cell High Content Screening in Drug Development
Milan Esner, Felix Meyenhofer, and Marc Bickle
?11. Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening
Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O’Connell, Martyn Banks, and Andrea Weston
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Part III: Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis
12. Translocation Biosensors - Versatile Tools To Probe Protein Functions In Living Cells
Verena Fetz, Roland H. Stauber, and Shirley K. Knauer
?13. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions
Yun Hua, Daniel P. Camarco, Christopher J. Strock, and Paul A. Johnston
?14. High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines
Paul A. Johnston, Malabika Sen, Yun Hua, Daniel Camarco, Tong Ying Shun, John S. Lazo, and Jennifer R. Grandis
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15. Single Cell and Population Level Analysis of HCA Data
David Novo, Kaya Ghosh, and Sean Burke
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16. Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens
?Judith Wardwell-Swanson and Yanhua Hu
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Section IV: Phenotypic Models ? Primary Neurons, Neural Stem/Progenitor Cells, Induced-Pluripotent Stem Cells, Multicellular Tumor Spheroids, Endothelial/Mesenchymal Stem Cell Co-Cultures, and Zebrafish Embryos
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17. High Content Screening of Mammalian Primary Cortical Neurons
Dario Motti, Murray Blackmore, John Bixby, and Vance Lemmon
?18. Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications
Joshua A. Harrill
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19. Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content AssaysOksana Sirenko and Evan F. Cromwell
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20. The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates
David A. Close, Daniel P. Camarco, Feng Shan, Stanton J. Kochanek, and Paul A. Johnston
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21. An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro
Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, and Mark Uhlik
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22. High-throughput Automated Chemical Screens in Zebrafish
Manush Saydmohammed and Michael Tsang
