Biodegradation of Cellulose: Enzymology and Biotechnology (Hardcover)

Chemistry and Structure of Cellulose and HeteroxylanCellulose - Heteroxylans and Associated Polymers: Composition and structure, Conformation and interactions of heteroxylan - Localization of Cellulose and Heteroxylans Enzymology of Biodegradation of Cellulose and HeteroxylansProperties of Cellulolytic and Heteroxylanolytic Enzymes: Cellulase, Cellobiohydrolase, beta-Glucosidase, Xylanase, beta-Xylosidase - Glycosylation of Cellulolytic and Xylanolytic Enzymes - Classification of beta-Glycosidases - Domain Structure: Cellulose binding domains, Linker sequences, Repeated sequences, Multi-catalytic domains Interactions and AssociationsMultiplicity - Synergy: Cellulolytic enzymes, Heteroxylanolytic enzymes - Cellulosomes Production and PurificationInduction: Cellulolytic enzymes, Xylanolytic enzymes, Heteroxylan side-chain hydrolases - Repression - Product Inhibition - Detection - Assay of Enzyme Activity: Cellulolytic enzymes, Xylanolytic enzymes - Production - Isolation: Fungi, Bacteria - Purification The Catalytic Mechanism of ActionStereochemistry - Catalytic Mechanism of Retaining and Inverting Glycosidases: Kinetics, Chemical modifications, Affinity labelling and mechanism-based inhibition, Mechanism of catalysis - Structure and Function Relationship of Catalytic Sites: Retaining enzymes, Inverting enzymes, Enzymes with uncharacterized stereochemistry Structure and Function RelationshipSubstrate-Binding Subsites - Identification of Binding Residues: Spectroscopy, Chemical modification, X-Ray crystallography - Modification of Catalytic Properties by Rational Design - Concluding Remarks