Protein Engineering Protocols (Hardcover)

Part I. Design and Computational Strategies for Protein Engineering Combinatorial Protein Design Strategies Using Computational Methods Hidetoshi Kono, Wei Wang, and Jeffery G. Saven Global Incorporation of Unnatural Amino Acids in Escherichia coli Jamie M. Bacher and Andrew D. Ellington Considerations in the Design and Optimization of Coiled Coil Structures Jody M. Mason, Kristian M. Muller, and Katja M. Arndt Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions Kevin Truong, Asako Sawano, Atsushi Miyawaki, and Mitsuhiko Ikura Design and Synthesis of Artificial Zinc Finger Proteins Wataru Nomura and Yukio Sugiura Monobodies: Antibody Mimics Based on the Scaffold of the Fibronectin Type III Domain Akiko Koide and Shohei Koide Engineering Site-Specific Endonucleases Peter Friedhoff and Alfred Pingoud Part II. Evolutionary Strategies for Protein Engineering Protein Library Design and Screening: Working Out the Probabilities Michel Denault and Joelle N. Pelletier Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids Luke H. Bradley, Yinan Wei, Peter Thumfort, Christine Wurth, and Michael H. Hecht Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology Sabine C. Stebel, Katja M. Arndt, and Kristian M. Muller Degenerate Oligonucleotide Gene Shuffling Peter L. Bergquist and Moreland D. Gibbs. M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display Sachdev S. Sidhu, Birte K. Feld, and Gregory A. Weiss Ribosome-Inactivation Display System Satoshi Fujita, Jing-Min Zhou, and Kazunari Taira Compartmentalized Self-Replication: A Novel Method for the Directed Evolution of Polymerases and Other Enzymes Farid J. Ghadessy and Philipp Holliger Synthesisof Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay Francois-Xavier Campbell-Valois and Stephen W. Michnick A General Method of Terminal Truncation, Evolution, and Re-Elongation to Generate Enzymes of Enhanced Stability Jochen Hecky, Jody M. Mason, Katja M. Arndt, and Kristian M. Muller Index