Transmembrane Signaling Protocols: (Hardcover, 2, 2006)

Part I. Overviews Transmembrane Signaling by G Protein-Coupled Receptors Louis M. Luttrell Crosstalk Coregulation Mechanisms of G Protein-Coupled Receptors and Receptor Tyrosine Kinases Kanchana Natarajan and Bradford C. Berk Protein-Protein Interactions in Signaling Cascades Bruce J. Mayer Part II. Specific Topics A. Membrane Receptors and Signaling Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells Marcin Majka and Mariusz Z. Ratajczak Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4 Zi-xuan Wang, James R. Broach, and Stephen C. Peiper Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors Jean-Marc Navenot, Zi-xuan Wang, and Stephen C. Peiper G Protein-Coupled Receptor Dimerization and Signaling Mario Mellado, Antonio Serrano, Carlos Martinez-A, and Jose Miguel Rodriguez-Frade Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cell Venkatakrishna R. Jala and Bodduluri Haribabu B. Lipid Raft and Caveolae in Transmembrane Signaling Isolation of Membrane Rafts and Signaling Complexes Kathleen Boesze-Battaglia Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae Rennolds S. Ostrom and Paul A. Insel C. Protein-Protein Interaction in Transmembrane Signaling Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions Tarik Issad and Ralf Jockers Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen Kelly L. Jordan-Sciutto and Marshall B. Montgomery Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays Hyun Woo Lee, Jaewon Ko, and EunjoonKim Clustering Assay for Studying the Interaction of Membrane Proteins With PDZ Domain Proteins Jaewon Ko and Eunjoon Kim D. Rho Guanosine Triphosphatases and Reconstitution of Signaling Complexes Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases Ritu Garg and Anne J. Ridley Affinity-Based Assay of Rho Guanosine Triphosphatase Activation Mary Stofega, Celine DerMardirossian, and Gary M. Bokoch Assay of Phospholipase D Activity in Cell-Free Systems Shankar S. Iyer and David J. Kusner Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D Amanda Fensome-Green and Shamshad Cockcroft E. Genomics and Proteomics Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors Fernando O. Martinez and Massimo Locati Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo Martha S. Jordan Proteomic Analysis of Human Neutrophils George Lominadze, Richard A. Ward, Jon B. Klein, and Kenneth R. McLeish Index