In Vitro Transcription and Translation Protocols (Hardcover, 2)

Section One: TECHNOLOGIES Chapter One - Energy Systems for ATP Regeneration in Cell-free Protein Synthesis Reactions Kara A. Calhoun, James R. Swartz Chapter 2 - Continuous-exchange Protein-synthesizing Systems Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin Chapter 3 - Cell-free production of integral membrane proteins on a preparative scale' Christian Klammt, Daniel Schwarz, Vilker Dotsch, Frank Bernhard Chapter 4- SIMPLEX: Single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano Chapter 5 - Methods for High-throughput Materialization of Genetic Information Based on Wheat Germ Cell-free Expression System Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo Chapter 6 - Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti Section Two: APPLICATIONS Chapter 7 -In vitro translation to study HIV protease activity Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi Chapter 8 - The protein truncation test in mutation detection and molecular diagnosis Oliver Hauss, Oliver Muller Chapter 9 - Creation of Novel Enantioselective lipases by SIMPLEX Yuichi Koga, Tsuneo Yamane, Hideo Nakano Chapter 10 - In vitro transcription and translation coupled to 2D-electrophoresis for bacterial proteome analysis Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi Chapter 11 - In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis Orit Gat, Haim Grosfeld,Avigdor Shafferman Chapter 12 - Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli Chapter 13 - Production of Protein for NMR Study Using the Wheat Germ Cell-Free System Toshiyuki Kohno, Yaeta Endo