RNAi and Plant Gene Function Analysis: Methods and Protocols (Hardcover)

1. Overview of Plant RNAi Yuichiro Watanabe 2. Caveat of RNAi in Plants: the Off-Target Effect Muthappa Senthil-Kumar and Kirankumar S. Mysore 3. Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies Toshiya Muranaka 4. Heat-Inducible RNAi for Gene Functional Analysis in Plants Frederic Masclaux and Jean-Philippe Galaud 5. Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens Basel Khraiwesh, Isam Fattash, M. Asif Arif, and Wolfgang Frank 6. Virus-Induced Gene Silencing in Ornamental Plants Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid 7. Local RNA Silencing Mediated by Agroinfiltration Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis 8. Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana Ha-il Jung, Zhiyang Zhai and Olena K. Vatamaniuk 9. Detection of Long and Short Double-Stranded RNAs Toshiyuki Fukuhara, Syunichi Urayama, Ryo Okada, Eri Kiyota, and Hiromitsu Moriyama 10. Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs Erika Varkonyi-Gasic and Roger P. Hellens 11. Large Scale Sequencing of Plant Small RNAs William P. Donovan, Yuanji Zhang, and Miya D. Howell 12. Computational Prediction of Plant miRNA Targets Ying-Hsuan Sun, Shanfa Lu, Rui Shi, and Vincent L. Chiang 13. Bisulfite S equencing for C ytosine- M ethylation A nalysis in Plants Nazmul Haque and Masamichi Nishiguchi 14. Using Nuclear Run-On Transcription Assays in RNAi Studies Basel Khraiwesh 15. Proteomic Analysis of RNA-Interference Induced Knockdown Plant Sang Yeol Lee and Kyun Oh Lee 16. Comparative Analysis of Phosphoprotein Expression using 2D-DIGE Tomoya Asano and Takumi Nishiuchi