Protein Blotting and Detection: Methods and Protocols (Hardcover)

Protein Blotting and Detection: Methods and Protocols Editors Biji T. Kurien, PhD and R. Hal Scofield, MD Table of Contents Preface- R. Hal Scofield and Biji T. Kurien Contributors 1. Origins of protein blotting- Dr. Harry Towbin 2. Western Blotting: Remembrance of Things Past-W. Neal Burnette 3. Introduction to protein blotting- Biji T. Kurien and R. Hal Scofield BLOTTING BY DIFFUSION 4. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis- Der-Yen Lee and Geen-Dong Chang 5. Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support- Ingrid Olsen and Harald G. Wiker 6. Tryptic peptide purification using polyvinylidene difluoride membrane for matrix assisted laser desorption ionization time of flight mass spectrometry -Biji T. Kurien and R. Hal Scofield 7. Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time-Biji T. Kurien and R. Hal Scofield 8. Non-electrophoretic bi-directional transfer of a single SDS-PAGE gel with multiple antigens to obtain twelve immunoblots - Biji T. Kurien and R. Hal Scofield 9. Slice blotting-Graeme Lowe 10. Localizing Proteins by Tissue Printing - Rafael Pont-Lezica 11. Dot-Immunobinding Assay (Dot-Iba) - Sumi Surendran, Annamma Mathai and Vishnampet Venkataraman Radhakrishnan 12. Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes after Agarose-gel Electrophoresis Separation- Nicola Volpi and Francesca Maccari MODIFICATIONS OF ELECTROBLOTTING 13. Quantitative computerized western blotting- Dalit Talmi-Frank, Charles L. Jaffe and Gad Baneth 14. Cationic electrophoresis and eastern blotting - Engelbert Buxbaum 15. A miniaturized blotting system for simultaneous detecting of different autoantibodies - Ulrich Canzler, Holger Bartsch, Kai Großmann, WernerLehmann, Karsten Conrad, Biji T. Kurien, Yaser Dorri, R. Hal Scofield, Michael P. Bachmann 16. Transfer and multiplex immunoblotting of a paraffin embedded tissue- Joon-Yong Chung and Stephen M. Hewitt 17. Multistrip Western Blotting to increase quantitative data output- Anatoly Kiyatkin and Edita Aksamitiene 18. The Western blotting/Edman sequencing technique using polyvinylidene difluoride (PVDF) membrane: Setsuko Komatsu 19. Blotting from PhastGel to membranes by ultrasound- Joseph Kost 20. Ultra-rapid electrophoretic transfer of high and low molecular weight proteins using heat- Biji T. Kurien and R. Hal Scofield 21. Strip immunoblotting of multiple antigenic peptides to nitrocellulose membrane- Biji T. Kurien and R. Hal Scofield 22. Affinity purification of autoantibodies from an antigen strip excised from a nitrocellulose protein blot - Biji T. Kurien and R. Hal Scofield 23. Double-blotting: A Solution to the Problem of Non-specific Binding of Secondary Antibodies in Immunoblotting Procedures - Francoise Lasne 24. Efficient electroblotting of very large proteins using a vertical agarose electrophoresis system- Marion L. Greaser and Chad M.