Protein Electrophoresis: Methods and Protocols (Hardcover, 2012)

'1. How It All Began -- A Personal History of Gel-Electrophoresis Oliver Smithies 2. Introduction to Protein Electrophoresis Pothur R. Srinivas 3. Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis J.P. Dean Goldring 4. Protein Solubilization: Attend to the Choice of Lysis Buffer Mandy Peach, Noelle Marsh, and Daniel J. MacPhee 5. Native Polyacrylamide Gels Claudia Arndt, Stefanie Koristka, Holger Bartsch, and Michael Bachmann 6. Cationic Electrophoresis Engelbert Buxbaum 7. SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins Christian Reichel 8. Tricine-SDS-PAGE Syed R. Haider, Helen J. Reid, and Barry L. Sharp 9. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen 10. Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins Marion L. Greaser and Chad M. Warren 11. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis Henghang Tsai and Hon-chiu Eastwood Leung 12. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann 13. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies Gyorgy Csako 14. Microchip Electrophoresis, with respect to "Profiling of Abeta Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease"Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy 15. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols Mustafa Celebier, Clara Ibanez, Carolina Simo, and Alejandro Cifuentes 16. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra 17. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa 18. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting Edward P. Trieu and Ira N. Targoff 19. Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing Yaser Dorri 20. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids Gyorgy Csako 21. Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution Biji T. Kurien and R. Hal Scofield 22. Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE Hiroyuki Matsumoto, Hisao Hanju, Biji T. Kurien, and Naoka Komori 23. One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta 24. Two-Dimensional Difference Gel Electrophoresis Jonathan S. Minden 25. Protein Identification on Archived 2-D Gels Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori 26. Diagonal Electrophoresis for the Detection of Protein Disulfides Brian McDonagh 27. Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes Jean Paul Lasserre and Armelle Menard 28. Immunoelectrophoresis: A Method with Many Faces Gyorgy Csako 29. Cell Surface Protein Biotinylation for SDS PAGE Analysis Giuliano Elia 30. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin 31. Gel Absorption-Based Sample Preparation Method for Shotgun