Protein Gel Detection and Imaging: Methods and Protocols (Hardcover, 2018)

Preface... Table of Contents... Contributing Authors... 1. Protein Stains and Applications Pazhani Sundaram 2. The Roles of Acetic Acid and Methanol during Fixing and Staining Proteins in a SDS Polyacrylamide Electrophoresis Gel J.P. Dean Goldring 3. Multicolored Prestained Standard Protein Marker Generation using a Variety of Remazol Dyes for Easy Visualization of Protein Bands during SDS-PAGE Gaurav Kumar 4. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, and Michael Bachmann 5. A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate Polyacrylamide Gel using Coomassie Blue Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, and Tian-yun Wang 6. Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue Biji T. Kurien and R. Hal Scofield 7. Silver Staining Techniques of Polyacrylamide Gels Nicole Berndt, Rolf Bergmann, Claudia Arndt, Stefanie Koristka, and Michael Bachmann 8. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry Sun-Yong Hwang and Jung-Kap Choi 9. Detection of Phosphoproteins in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis using 8-Quinolinol Stain Sun-Yong Hwang, Xu Wang, and Jung-Kap Choi 10. Microwave Assisted Protein Staining, De-Staining and In-Gel/In-Solution Digestion of Proteins Jennie R. Lill and Victor J. Nesatyy 11. Fluorescent Staining of Gels Engelbert Buxbaum 12. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta during Western Blot Analysis Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni 13. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE Na Na and Jin Ouyang 14. Detection of Glycoproteins in Polyacrylamide Gels using Pro-Q Emerald 300 Dye, A Fluorescent Periodate Schiff-Base Stain Padmaja Mehta-D'souza 15. Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain Biji T. Kurien, Yaser Dorri, and R. Hal Scofield 16. Detection of Multiple Enzymes in Fermentation Broth using Single PAGE Analysis Divakar, K., Deepa Arul Priya, J, Panneer Selvam, G., Suryia Prabha, M., Ashwin Kannan, Nandhini Devi, G., and Pennathur Gautam 17. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis Han-Min Chen 18. A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins Jelena Bogdanovic Pristov and Ivan Spasojevic 19. Tem Minute stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct red 81 and Amido Black J.P. Dean Goldring and Robert G.E. Krause 20. In-Gel Protein Phosphatase Assay using Fluorogenic Substrates Isamu Kameshita, Noriyuki Sueyoshi, and atsuhiko Ishida 21. Detection of Proteins in Polyacrylamide Gels via Pre-Labeling by Isatoic Anhydride Kazem Asadollahi, Saharnaz Rafiee, and Gholamhossein Riazi 22. Fluorescent Protein Visualization Immediately after Gel Electrophoresis using an In-Gel Trichloroethanol Photoreaction with Tryptophan Carol L. Ladner-Keay, Raymond J. Turner, and Robert A. Edwards 23. Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend 24. Zymographic Determination of Intrinsic Specific Activity of Oxidases in Presence of Interfering Proteins Tien Canh Le, Mircea Alexandru Mateescu, Samaneh Ahmadifar, Lucia Marcocci, and Paola Pietrangeli 25. A Simple Method for Detecting Phosphorylation of Proteins by using ZN2+ -Phos-tag SDS-PAGE at Neutral pH Gaurav Kumar 26. Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Gaurav