Life Science (Hardcover)

Life Science.- Cryoelectron microscopy: from molecules to systems.- Progress in High-resolution Scanning Probe Microscopy.- Analysis of macromolecules and their supramolecular assemblies.- Modular organization of RNase P.- Molecular anatomy of the human pathogen Leptospira interrogans.- Unveiling conformational changes of biological molecules using multiscale modeling and multiresolution experiments.- Nicolas Boisset: In memoriam.- Cryo TEM-based 3D reconstruction of the recombinant expressed human zinc peptidase Meprin ?.- Origin melting by SV40 Large T antigen-new insights from 3D-EM-MLF3D classification method.- Sequence analysis and modelling of the two large subunits of Phosphorylase Kinase.- Cellular Uptake of Polymer Nanoparticles Imaged by Electron Microscopy.- 9 A cryo-EM structure and molecular model of a gastropod hemocyanin didecamer (KLH1) reveals the architecture of the asymmetric collar.- Microstructure of model systems for sauces based in polysacharides observed by Cryo-SEM.- Three-dimensional architecture of outer- and inner-dynein arms in flagella revealed by cryo-electron tomography and single particle analysis.- Structural and functional studies of rabbit skeletal muscle Phosphorylase Kinase.- Single-particle analysis of the Cdc6/Orc1, archaeal DNA replication initiator protein.- A high-throughput acquisition toolbox in Matlab for cryo-electron microscopy studies.- Architecture of bacterial glutamate synthase complexe using hybrid approaches.- 3D Structure of DNA Repair Macromolecular Complexes Participating in Non-Homologous End-Joining (NHEJ).- Quaternary structure of recombinant human meprin ?, a zinc peptidase of the astacin family.- Allosterism of Nautilus pompilius hemocyanin as deduced from 8 A cryo-EM structures obtained under oxy and deoxy conditions.- High resolution structure of a 6 MDa protease by xray-crystallography and cryo-EM.- Cryo-TEM of liquid crystalline particles: Application of materials science techniques to study liquid crystalline particle structures.- Structural basis for the concerted integration of HIV-1 DNA in the human genome, role of the human cofactor LEDGF/p75.- Titan Krios: Automated 3D imaging at ambient and cryogenic conditions.- Self assembly and phase behaviour of new sugar based gemini amphiphiles.- Peptide-nanotube formation by lysine based lipoamino acids.- Advances in biological microanalysis using event streamed spectrum imaging and programmed beam acquisition.- 3D-correlation-averaging for membrane-protein-crystals.- 8 A cryo-EM structure of the giant hemoglobin from the planorbid snail Biomphalaria glabrata.- Structure of Pex5p and Pex5-20 complexes in the yeast Hansenula polymorpha. Pex20p causes a conformational change upon binding to Pex5p tetramers involved in peroxisomal protein transport.- Structure of OprM-MexA interacting complex revealed by cryo electron tomography.- Cell Structure and Dynamics.- Pleiomorphic viruses revealed by cryo tomography: the structure of coronaviruses.- Combined structural and chemical analysis of unique anammox bacteria that contain a prokaryotic organelle.- Structural and functional considerations on the 3-D organization of the fenestrated cytoplasm of hepatic endothelial cells.- Molecular architecture of the presynaptic compartment studied by cryo-electron tomography.- Synapses in high pressure frozen Caenorhabditis elegans.- High resolution EM-tomography of a whole vitrified cell.- Evaluation of beam damage in catalase crystals observed in vitrified sections.- Urothelial fusiform vesicles are formed in post-Golgi compartment.- Localisation of GFP and GFP tagged PKD2 in cultivated pancreatic cancer cells using high-pressure freezing.- Cytomegalovirus membrane envelopment revisited ? a STEM-tomography study based on high-pressure freezing and freeze substitution.- Electron tomography & template matching of biological membranes.- Correlated Microscopy: From Dynamics to Structure.- 3DEM-analyses of Golgi apparatus subunits during physiologic and pathologic reorganizations.- Visualization of the 80s ribosome in situ using cryo electron tomography of vitreous sections.- Three-dimensional analysis of the intermediate filament network using SEM-tomography.- The apical vesicles of the tuft cells in the main excretory duct of the rat submandibular gland by EFTEM-TEM tomography.- (S)TEM Dual Axis Tomography.- STEM tomography of high-pressure frozen cell monolayers.- Multimodality of pericellular proteolysis in cancer cell invasion.- Photonic crystal type nanoarchitectures in butterfly wing scales.- Iridovirus-like viruses of Lacerta monticola from Serra da Estrela, Portugal.- Structural and ultrastructural investigations on the in vitro effects of plant extracts in chicken and rabbit whole blood cultures.- Fine structural characterization of cloned human endogenous retrovirus HERV-K113.- The procaine hydrochlorate effect onto the corpuscular anthocyans from the vacuolar sap of different plant cells.- Comparative anatomical and ultrastructural investigations on normal and vitroplantlets leaves from Pistia stratiotes L..- Autophagy of mitochondria in brown adipocytes of chemically thyroidectomised rats.- Ultrastructural studies of immune organs in antigen primed chickens treated with vegetal extractions.- Structural features of HIV-1 immunologic activation.- Functions of actin-binding proteins in the cell nucleus.- Ultrastructural mechanisms of entamoebae movement system.- White adipocytes transdifferentiation into brown adipocytes induced by triiodothyronine.- Ultrastructural studies of the tegument of cestodes (Platyhelminthes): phylogenetic implications.- Crystallization stages of the CaCO3 deposits in the earthworm’s calciferous gland.- Scanning electron microscopic analysis of heavy metal resistant microorganisms.- Characterization of mouse embryoid bodies by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM).- Stage-dependent localization of mitochondrial DNA during the cell cycle of Euglena gracilis Z by immunogold electron microscopy.- The virus-inducted structures in RNA virus infected macrophages.- Surface layers of ore-leaching Bacteria and Archaea.- Structure of Bacillus anthracis spores analyzed by CEMOVIS.- Insights into the role of pUL71 during HCMV morphogenesis.- Fine structure of the genital chamber of female Chrysomya megacephala (F.) (Diptera: Calliphoridae).- Ultrastructure of the testes and spermatozoa of the blow fly Chrysomya megacephala (F.) (Diptera:Calliphoridae).- Biosynthesis of cellulose in red algae.- Light and electron microscopy aspects of the glandular sessile hairs from the vitroplantlet leave of Drosera rotundifolia.- Ultrastructure of selected Euglena species in relation to their taxonomy.- Confocal Microscopy Reveals Molecular Interactions Between DNA-binding Drugs and Chromatin in Live Cells.- Fluorescence microscopy methods for measuring the mobility and stability of molecules in 3-D samples.- Phasor-FLIM analysis of FRET for homotypic and heterotypic non-covalent interactions: stimulated membrane receptors in live cells.- In vitro immunofluorescence and ultrastructural analysis of the expression of noncollagenous matrix proteins in human pulpal cells induced by mineralizing factors.- Differentiation-dependent Golgi fragmentation in the bladder urothelial cells in vitro.- Monitoring Mitochordria Dynamics in Living Cell System by Confocal Microscopy.- The freeze-fracture replica immunolabeling technique for the study of lipid droplet biogenesis and function.- Dynamics of epigenome duplication, accessibility and translation.- Controlled light exposure microscopy reveals telomeric microterritories throughout the cell cycle.- Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic.- Spectral methods for cells and particles identifications.- 3D realistic visualization of supramolecular assemblies.- Heterosis in Arabidopsis thaliana: Structural aspects in mature and germinating seeds from hybrid and parental lines.- Colour visualization of red blood cells in native smears by the new method reflected light microscopy.- In vitro culture of Trigonella foenum-graecum plantules and their anatomic characterization.- Live cell imaging by SEM-hosted X-ray microscope in “water window” energy range.- Microscopic anatomy and mineral composition of cuticle in amphibious isopods Ligia italica and Titanethes albus (Crustacea:Isopoda).- In vivo imaging and quantification of the continuous keratin filament network turnover.- Microscopy advances in the life sciences.- Necrotic cell death, a controlled way of cellular explosion.- Dissecting mitochondrial protein distributions using sub-diffraction resolution fluorescence microscopy.- High Thoughput, High Content Tissue Cyotometry.- Focus on the vascular wall: Imaging of large arteries using two-photon microscopy.- High resolution confocal Ca2+ imaging of the pulmonary neuroepithelial body microenvironment in lung slices.- Two photon excitation microscopy and SHG imaging as a tool for visualisation of type I and type II collagen, and their use in tissue engineering.- Testing calibration standards for confocal and two-photon microscopy.- Distribution of the voltage-gated delayed-rectifier K+ subunits, Kv1.1 and Kv1.2, in the adult murine enteric nervous system.- Observation of Ventricular Myocyte Morphology in Long Term Culture using High Resolution Confocal Imaging.- A magnetic field enhances the inmunofluorescence signal in a lymphocytic cell.- Novel 515 nm DPSS-laser brings excitement in life cell imaging.- Confocal laser scanning microscopy study on human fissure caries stained with alizarin red.- Non-invasive skin tissue characterization using non-linear spectral imaging microscopy.- Force Spectroscopy reveals mechanical structure of capsids.- Manipulating and imaging molecular motors with optical traps, single-molecule fluorescence and atomic force microscopy.- Mechanical stability of ethanol-induced A-DNA.- Manipulation of the mechanical properties of a virus by protein engineering.- A single-molecule mechanical assay to study DNA replication coupled to DNA unwinding.- Generation of multiple DNA lesions at subnuclear resolution by multi-photon irradiation.- Light and electron microscopy of phagosomes.- The Use of Electron Microscopy in the Diagnosis of Human Microsporidial Infection ? the Manchester, UK, Experience.- Overview of the impact of biological microanalysis in health and disease.- Immunolocalization of FOXP3 in HCV-infected liver biopsies. Preliminary observations..- Ultrastructural and Intracellular Elemental Composition Analysis of Human Hematopoietic Cells During Cold Storage in Preservation Solutions.- Reconstitution and morphological characterisation of an original Human Endogenous Retrovirus.- Cell death in human articular chondrocyte: an ultrastructural study in micromass model..- Pathomorphological diagnostic of paraffin embedded versus epon embedded cardiac tissues with transmission electron microscope analysis.- Interaction of carbon nanotubes with macrophages: STEM and EELS approach.- The ultastructural effect of nitrogen mustard gas on rat brain and therapeutic value of proanthocyanidin.- Assembly and maturation of pestiviruses.- Analysis of radiation-induced bystander effects using high content screening.- Electron microscopic investigations on normal and dexamethasone applied rat placentas.- Changes in dental enamel birefringence after CO2 laser irradiation through fluoride gel -a pilot study.- SEM and light microscopic examination and morphometric analysis of temporal changes in intimal and medial connective tissue component of human carotid arteries.- Ultrastructure of human amniotic membrane covering villous chorion and smooth chorion in preeclampsia.- Changes in Intracellular Sodium, Chlorine, and Potassium Content in Hematopoietic Cells after Hypotermic Storage.- Requirements for a bright future of electron microscopy in the rapid laboratory diagnosis of infectious diseases.- Changed thickness of zona pellucida as a result of stimulated ovulation.- Mozaic microscopy of pancreatic islets.- Microscope history Database.- Interaction of H-Ras Transformed Cell Line with Folic Acid Modified Magnetic Nanoparticles and Detection by Transmission Electron Microscope.- Sedimentation of suspensions for diagnostic thin section electron microscopy.- Evaluation of rofecoxib administration influence on ultrastructural image of the kidney.- Microstructural changes produced by Pulsed Electric Fields in liquid whole egg studied by Transmission Electron Microscopy (TEM).- Microstructural changes in dough treated by Glucose oxidase (GOX) and Transglutaminase (TG), studied by Scanning Electron Microscopy (SEM).- Cytoplasmic K/Na balance in the cardiomyocyte of young and old rats.- Hyperspectral imaging ? a novel concept for marker free chromosome characterization.- Poxviruses: Morphogenesis of an Orthopoxvirus (CPXV) and an Avipoxvirus (FPXV) in host tissue.- Ultrastructural alterations of methotrexate in mouse kidney.- Ultrastructural gadolinium evidence in skin of a patient with Nephrogenic Fibrosing Dermopathy.- Nephrotoxicity induced by inorganic Hg(II) and Pb(II): a microscopic and biochemical in vitro study.- Tissue distribution of peroxisomes in zebrafish.- An analysis of the ultrastructure of matured HIV.- Trichinella spiralis and Trichinella britovi cuticle and hypodermal glands Ultrastructure.- Ultrastructural analysis of lysosomal storage diseases: effects of therapy.- Sample preparation and identification of molecular targets.- Immunolabeling for Electronmicroscopy.- Molecular Organisation of Cadherins in Intercellular Adhesion Junctions by Cryo-Electron Tomography of Vitreous Sections.- Ultrastructural observations of indium in the lactating mammary gland cells.- An active mechanism flanks and modulates the export of the small ribosomal subunits.- New methods for micro-domain detection in bacterial sacculi.- Subcellular localization of Myogenic Regulatory Factors along skeletal muscle development.- Immunocytochemical Strategies: LR Resins or Lowicryl, Gold or Peroxidase ? Which is Better?.- Elemental Analysis in Electron Microscopy for Medical Diagnostics.- Synaptic localization of KCa1.1 potassium channels in central neurons revealed by postembedding immunogold and SDS-digested freeze-fracture replica labelling.- Ultrastructural studies of rod photoreceptor nuclei from SCA7 mouse.- Application of electron energy loss spectroscopy for heavy metal localization in unicellular algae.- Localization of “flagella” proteins in the Archaeon Ignicoccus hospitalis.- Detection of gold nanoparticles by Autrata YAG detector in FE SEM working in cryo mode.- Correlative cryo-fluorescence and electron microscopy.- The effect of hypometabolizing molecules on transcription as shown by a new two-step pulse-chase method.- Cryofixation and Freeze-Substitution Combined with Tokuyasu Cryo-section Labelling.- The Drosophila active zone architecture: combining confocal, STED and transmission electron microscopy.- Distribution and function of amorphous CaCO3 and calcite within the tergite cuticle of terrestrial isopods (Crustacea).- A symbiosis: tracking cell signaling with expression probes, quantum dots and a programmable array microscope (PAM).- Golgi twins in mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy.- Understanding the 3D architecture of organelle bound protein complexes using cryo-electron tomography of frozen hydrated sections and immunogold; our next great challenge.- Cryo-electron microscopy of vitreous sections.- The visualization of viruses in the low-voltage electron microscope.- Exploring HPF/FS methods for sensitive immunogold labeling on resin sections.- Low contrast of the ER-membranes in the high pressure frozen, freeze substituted specimens.- HPF of cultured cell monolayers: towards a standard method for high pressure freezing, freeze substitution and electron tomography acquisition.- Life-like physical fixation of large samples for correlative microscopy.- Integrating automated single, double and triple in situ molecular detection with imaging for the analysis of inter- and intracellular events in the seminiferous tubule.- Freeze-substitution in Epon: An attempt to combine immunolabeling and improvement of structural preservation.- The Shape of Caveolae is Omega-like after Chemical Fixation and Cup-like after Cryofixation.- Hybrid methods and approaches in microscopy.- Integrated laser and transmission electron microscopy.- Correlative 3D microscopy: CLSM and FIB/SEM tomography used to study cellular entry of vaccinia virus.- Strategies for the morphological analysis of hydrated and life-like preserved biomedical material in the SEM.- Denaturation of metaphase chromatin plates observed by transmission electron microscopy and atomic force microscopy in aqueous solution.- Discovery of a Nuclear Structure in UVC-induced Apoptotic Cells by Integrated Laser Electron Microscopy.- Analysis of biomineral formation in three-dimensional micro-mass stem cell cultures.- Comparative structural studies of highly-filled polymethacrylates with light microscopy, SEM, TEM and AFM.- Cell surface characteristics as reporters for cellular energy state.- Analysis of multimodal 3D microscopy measurements.- Permanent plastid ? nuclear complexes (PNCs) in plant cells.- Cell imaging by dynamic secondary ion mass spectrometry (SIMS): basic principles and biological applications.- Water imaging in cells cryosections by electron energy loss spectroscopy.- Study of the cellular uptake of Pt nanoparticles in human colon carcinoma cells.- Ion spectrometry and electron microscopy correlative imaging for accurate comprehensive trace element localization at subcellular level, in normal and pathological keratinocytes.- Calcium Spark Detection and Analysis in Time Series of Two-Dimensional Confocal Images.- Visualisation of the attachment, possible uptake and distribution of technical nanoparticles in cells with electron microscopy methods.