식물검역 바이러스 검사법

PART 1. 식물검역 바이러스 검사 매뉴얼
I. 검사 절차: 종자&구근, 묘, 묘목 3
II. Probe real-time PCR 검사법을 이용한 실험 및 결과 분석 5
III. RT&Nested-PCR 검사법을 이용한 실험 9
IV. Multiplex RT&Nested-PCR 검사법을 이용한 실험 11
V. PCR 검사법 결과 분석 13

PART 2. Probe real-time PCR 검사법
I. 연구사업 보고서 17
II. 식물검역 바이러스 36종의 probe real-time PCR 검사법 개발 결과
1. APLV Andean potato latent virus 31
2. APMoV Andean potato mottle virus 32
3. ArMV Arabis mosaic virus 33
4. BCMNV Bean common mosaic necrosis virus 34
5. BPMV Bean pod mottle virus 35
6. CGMMV Cucumber green mottle mosaic virus 36
7. CLRV Cherry leaf roll virus 37
8. CNFV Carnation necrotic fleck virus 38
9. CNRMV Cherry necrotic rusty mottle virus 39
10. CPMMV Cowpea mild mottle virus 40
11. CRLV Cherry rasp leaf virus 41
12. CRSV Carnation ringspot virus 42
13. FoMV Foxtail mosaic virus 43
14. GFLV Grapevine fanleaf virus 44
15. INSV Impatiens necrotic spot virus 45
16. IYSV Iris yellow spot virus 46
17. KGMMV Kyuri green mottle mosaic virus 47
18. LRSV Lychnis ringspot virus 48
19. PDV Prune dwarf virus 49
20. PEMV Pea enation mosaic virus 50
21. PepMV Pepino mosaic virus 51
22. PMTV Potato mop-top virus 52
23. PRMV Peach rosette mosaic virus 53
24. PSTVd Potato spindle tuber viroid 54
25. PZSV Pelargonium zonate spot virus 55
26. RpRSV Raspberry ringspot virus 56
27. SBMV Southern bean mosaic virus 57
28. SLRSV Strawberry latent ringspot virus 58
29. SqMV Squash mosaic virus 59
30. TBRV Tomato black ring virus 60
31. TBSV Tomato bushy stunt virus 61
32. ToRSV Tomato ringspot virus 62
33. TRSV Tobacco ringspot virus 63
34. TSV Tobacco streak virus 64
35. TYMV Turnip yellow mosaic virus 65
36. WSMV Wheat streak mosaic virus 66
III. Probe real-time PCR 검사법 troubleshoot 67
IV. 부록
Probe real-time PCR 검사법의 primer, probe 및 염기서열 정보 72

PART 3. RT&Nested-PCR 검사법
I. 연구사업 보고서 87
II. 식물검역 바이러스 37종의 RT&Nested-PCR 검사법 개발 결과
1. APLV Andean potato latent virus 105
2. APMoV Andean potato mottle virus 113
3. ArMV Arabis mosaic virus 124
4. BCMNV Bean common mosaic necrosis virus 131
5. BPMV Bean pod mottle virus 142
6. CCMV Cowpea chlorotic mottle virus 154
7. CGMMV Cucumber green mottle mosaic virus 164
8. CLRV Cherry leaf roll virus 171
9. CNFV Carnation necrotic fleck virus 181
10. CNRMV Cherry necrotic rusty mottle virus 190
11. CPMMV Cowpea mild mottle virus 193
12. CRLV Cherry rasp leaf virus 200
13. CRSV Carnation ringspot virus 206
14. CtRLV Carrot red leaf virus 217
15. FoMV Foxtail mosaic virus 221
16. GFLV Grapevine fanleaf virus 225
17. IYSV Iris yellow spot virus 237
18. KGMMV Kyuri green mottle mosaic virus 247
19. LRSV Lychnis ringspot virus 254
20. PCV Peanut clum virus 261
21. PDV Prune dwarf virus 269
22. PEMV Pea enation mosaic virus 282
23. PepMV Pepino mosaic virus 289
24. PMTV Potato mop-top virus 298
25. PRMV Peach rosette mosaic virus 307
26. PSTVd Potato spindle tuber viroid 317
27. PZSV Pelargonium zonate spot virus 324
28. RpRSV Raspberry ringspot virus 331
29. SBMV Southern bean mosaic virus 345
30. SLRSV Strawberry latent ringspot virus 352
31. SqMV Squash mosaic virus 362
32. TBRV Tomato black ring virus 369
33. TBSV Tomato bushy stunt virus 378
34. ToRSV Tomato ringspot virus 385
35. TRSV Tobacco ringspot virus 397
36. TSV Tobacco streak virus 408
37. WSMV Wheat streak mosaic virus 416
III. 부록
바이러스별 primer, PCR product 사이즈 및 돌연변이 양성대조구의
염기서열 정보

PART 4. Multiplex RT&Nested-PCR 검사법
I. 연구사업 보고서 447
II. 5품목의 multiplex RT&Nested-PCR 검사법 개발 결과
1. 박과 461
2. 페튜니아 468
3. 상추 475
4. 고추&토마토 A 482
5. 토마토 B 490