Sphingolipid Metabolism and Cell Signaling, Part B: Volume 312 (Hardcover)

Section I: Methods for Analyzing Sphingolipids

[1]: Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography

[2]: Enzymatic Method for Measurement of Sphingosine 1-Phosphate

[3]: Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography

[4]: Quantitative Determination of Ceramide Using Diglyceride Kinase

[5]: Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass Spectrometry

[6]: Analyses of Glycosphingollpids by High-Performance Liquid Chromatography

[7]: Sphingolipid Extraction and Analysis by Thin-Layer Chromatography

[8]: Extraction and Analysis of Multiple Sphingolipids from a Single Sample

[9]: Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges

[10]: Ganglioside Analysis by High-Performance Thin-Layer Chromatography

[11]: Purification and Analysis of Gangliosides

[12]: Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications

[13]: Thin-Layer Chromatography Immunostaining

[14]: Monoclonal Anti-Glycosphingolipid Antibodies

[15]: Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies

[16]: Cloud-Point Extraction of Gangliosides using Nonionic Detergent C14EO6

[17]: Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase

[18]: Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance

[19]: Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases

[20]: Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models

[21]: Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics

[22]: Fluorescence Quenching Assay of Sphingolipid/Phospholipid Phase Separation in Model Membranes

Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells

[23]: Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells

[24]: Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis

[25]: Selection of Yeast Mutants in Sphingolipid Metabolism

[26]: Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression

[27]: Mammalian Ganglioside Sialidases: Preparation and Activity Assays

Section III: Sphingolipid-Protein Interactions and Cellular Targets

[28]: Effects of Sphingosine and Other Sphingolipids on Protein Kinase C

[29]: Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase

[30]: Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein

[31]: Synthesis and Use of Caged Sphingolipids

[32]: Binding of Sphingosine 1-Phosphate to Cell Surface Receptors

[33]: Use of Short-Chain Ceramides

[34]: Analysis of Ceramide-Activated Protein Phosphatases

[35]: Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of Ceramide-Binding Proteins

[36]: Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids

[37]: Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate-Carbohydrate Interaction

[38]: Analysis of Interactions between Glycosphingolipids and Microbial Toxins

[39]: Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Soluble Glycoconjugates

[40]: Separation of Glycosphingolipid-Enriched Microdomains from Caveolar Membrane Characterized by Presence of Caveolin

[41]: Reconstitution of Sphingolipid-Cholesterol Plasma Membrane Mlcrodomalns for Studies of Virus-Glycolipid Interactions

[42]: Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Saccharomyces cerevisiae

[43]: Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecules

Section IV: Sphingolipid Transport and Trafficking

[44]: Applications of BODIPY-Sphingolipid Analogs to Study Lipid Traffic and Metabolism in Cells

[45]: Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immunoelectron Microscopy

[46]: Assays for Transmembrane Movement of Sphingolipids

Section V: Other Methods

[47]: Compilation of Methods Published in Previous Volumes of Methods in Enzymology

Author Index

Subject Index