Glycomics: Volume 416 (Hardcover)

Section 1 - Carbohydrate-Protein Interactions-Physical Measurements

Characterization of specific donor binding to ￡\1,4-N-acteylhexosaminyltransferase EXTL2 using Isothermal Titration Calorimetry.
NMR Analysis of Carbohydrate-Protein Interactions.
Determination of atomic structure for glycosyltransferase-substrate complex.

Section 2 ¡V Phage Display Library

Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates.
Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan epitopes.

Section 3 - Glycosyltransferase Expression Profiling

Autofluorescent proteins for monitoring the intracellular distribution of glycosyltransferases.
Expression profiling of glycosyltransferases and their related enzymes using in situ hybridization.
Expression profiling of glycosyltransferases using RT-PCR.
Expression profiling of lycosyltransferases and related enzymes using gene micro-arrays.

Section 4 - CHO mutants in Glycan Biosynthesis

Lectin Resistant CHO Glycosylation Mutants.
CHO glycosylation mutants ¡V GPI anchor.
CHO glycosylation mutants ¡Vproteoglycans.

Section 5 - Function of Sulfation

Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Protoglycans).
Measuring the activities of the sulfs, two novel heparin/heparan sulfate endosulfatases.
Determination of chemokine-glycosaminoglycan interaction specificity.
Identification of Proteoglycan-binding Proteins.
Functions of glycans revealed by gene inactivation of L-selectin ligand sulfotransferases in mice.


Section 6 - Functions Revealed by Forced Expression or Gene Knockout

Expression of specific carbohydrates by transfection with specific carbohydrate modifying enzymes.
N-glycan structure analysis using lectins and an a-mannosidase IIx activity assay.
Targeted disruption of the gene encoding core 1 ?O1-3-galactosyltransferase (T-synthase) causes embryonic lethality and defective angiogenesis in mice.
Roles of Mucin-Type O-glycans Synthesized by Core2B1,6-N-aetylglucosaminyltransferase.
Analysis of leukocyte rolling in vivo and in vitro.
Cell type-dependent Roles of Carbohydrates in Tumor Metastasis.