Pcr Protocols (Hardcover)

[Contents in a condensed version]
Basic Methodology
Amplification of genomic DNA targets
Optimization of PCR reaction and design of primers
Nonradioactive detection of PCR products
mRNA quantitation by PCR
Cloning PCR products
Contamination and product carry-over
PCR from paraffin sections
Amplifying ancient DNA
Research Applications
Direct DNA sequencing of PCR products
DNA footprinting
Detection of rare mRNAs
Site-directed mutagenesis
Screening DNA libraries
Amplification of flanking DNA sequences
Genetics and Evoltion
Tissue typing and disease susceptibility
Haplotype analysis of single cells
Population biology
Multiplex DNA amplification
Diagnostics and Forensics
HIV detection
HTLV I and II detection
Screening for novel viruses
Detection of oncogenic mutations
Diagnosis of retinoblastoma
Individual identification
Instruments and Supplies
Organizing a laboratory for PCR work
Low-cost air-driven cycling oven
Basic equipment and supplies for the PCR lab