Crispr-Cas Enzymes: Volume 616 (Hardcover)

1. CRISPR bioinformatics 2. A method for one-step assembly of Class 2 CRISPR arrays 3. Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems 4. Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system 5. Site-specific fluorescent labeling of individual proteins within CRISPR complexes 6. Fluorescence-based methods for measuring target interference by CRISPR-Cas systems 7. Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry 8. ChIP-based assays to measure Cas protein binding to DNA in vivo 9. Live-cell super-resolution microscopy of Cascade-mediated DNA surveillance 10. In vitro assembly of Crispr-Cas Type III-A system 11. Cyclic oligoadenylate signalling by type III CRISPR systems 12. A pipeline for characterization of Cas9 orthologues 13. Preparation and use of Cas12a RNPs for genome editing experiments 14. Discovery and Mechanistic Studies of A Thermophilic Cas9 towards Gene Editing and Cancer Diagnostics Applications 15. Kinetic characterization of Cas9 enzymes 16. Single-molecule FRET studies of Cas9 endonuclease 17. CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA 18. dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) 19. CRISPR-Cas10 assisted editing of virulent bacteriophages 20. Reconstituted in vitro CRISPR adaptation and its detection by PCR 21. Quantitative deep sequencing of CRISPR-Cas spacer acquisition