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· 제목 : TET Proteins and DNA Demethylation: Methods and Protocols (Paperback)
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 생화학
· ISBN : 9781071612965
· 쪽수 : 320쪽
· 출판일 : 2021-05-20
· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 생화학
· ISBN : 9781071612965
· 쪽수 : 320쪽
· 출판일 : 2021-05-20
목차
Preface…
Table of Contents…
Contributing Authors…
Part I Detection and Quantification of DNA Methylation Turnover
1) Reduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine
Michael J. Booth and Shankar Balasubramanian
2) Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine
Zhiyi Sun, Jolyon Terragni, Zhenyu Zhu, Yu Zheng, and Sriharsa Pradhan
3) Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome Bisulfite Sequencing (WGBS)
Oscar Ortega-Recalde, Julian R. Peat, Donna M. Bond, and Timothy A. Hore
4) ELISA-Based Quantitation of Global 5hmC Levels
Nelly N. Olova
5) Avidin-Biotin ELISA-Based Detection of 5hmC
Nelly N. Olova
6) Quantification of DNA Methylation and Its Oxidized Derivatives using LC-MS
Franziska R. Traube, Sarah Schiffers, and Thomas Carell
Part II Bioinformatic Analysis of DNA Methylation and Hydroxymethylation
Rochelle L. Tiedemann, Hope E. Eden, Zhijun Huang, Keith D. Robertson, and Scott B. Rothbart
8) Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions
Samara Flamini Kiihl
9) TAB-seq and ACE-seq Data Processing for Genome-Wide DNA Hydroxymethylation Profiling
Ksenia Skvortsova and Ozren Bogdanovic
Part III Precise Epigenome Manipulation using TET Enzymes
10) Genomic Targeting of TET Activity for Targeted Demethylation using CRISPR/Cas9
Trung Viet Nguyen and Ryan Lister
11) High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation
Xingbo Xu and Elisabeth M. Zeisberg
Part IV TET and DNA Hydroxymethylation Biochemistry
12) Identifying Protein?(hydroxy)methylated DNA Interactions using Quantitative Interaction Proteomics
Velin Marita Sequeira and Michiel Vermeulen
13) Purification of TET Proteins
Zhijun Huang, Jiyoung Yu, Jennifer Johnson, Seung-Gi Jin, and Gerd P. Pfeifer
Tian V. Tian and Jose Luis Sardina
15) ChIP-Sequencing of TET Proteins
Kasper D. Rasmussen and Kristian Helin
Part V Assessing TET Protein Activity and Function
16) Harnessing Alternative Substrates to Probe TET Family Enzymes
Uday Ghanty, Juan C. Serrano, and Rahul M. Kohli
17) Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts
Samuel E. Ross and Ozren Bogdanovic
Subject Index List…
