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· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 분자 생물학
· ISBN : 9781071624364
· 쪽수 : 363쪽
· 출판일 : 2022-07-26
목차
1. Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens
Oriana Genolet, Liat Ravid Lustig, and Edda G. Schulz
2. Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions
Taha Bartu Hayal and Ay?egul Do?an
3. Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish
Fatima Bianca A. Dessouki, Pawan K. Singal, and Dinender K. Singla
4. Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells
Federico Pecori and Shoko Nishihara
5. High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model
Olga Gordeeva
6. Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications
Loriana Vitillo and Ludovic Vallier
7. Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol
Hatice Burcu ?i?li, Selinay ?enkal, Derya Sa?rac, Taha Bartu Hayal, and Ay?egul Do?an
8. ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells
Divya Desai and Prasad Pethe
9. Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids
Yoshitomo Ueda, Stephen T. Moore, and Eri Hashino
10. Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs)
Bibiana Correia, Maria Ines Sousa, and Joao Ramalho-Santos
11. Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Arzuhan Koc and Esra Cagavi
12. Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo
Katsuhiro Konno, Jingjing Kobayashi-Sun, Fumio Arai, Isao Kobayashi, and Daisuke Sugiyama
13. Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells
Wen Huang, Sze Wan Fong, William Shu Biu Yeung, and Yin-Lau Lee
14. Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions
Luciana Isaja, Sofia Lujan Ferriol-Laffouillere, Sofia Mucci, Maria Soledad Rodriguez-Varela, and Leonardo Romorini
15. A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells
Helena Covelo-Molares, Yara Souto, Miguel Fidalgo, and Diana Guallar
16. Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation
Magdalena Guerra-Crespo, Omar Collazo-Navarrete, Rodrigo Ramos-Acevedo, Carmen Alejandra Morato-Torres, and Birgitt Schule
17. A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation
Jianqun Zheng, Ying Ye, Qiushi Xu, Wei Xu, Wensheng Zhang, and Xi Chen
18. Osteogenic Differentiation from Mouse Embryonic Stem Cells
Zahra Alvandi and Michal Opas
19. Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation
Manar Elkenani and Belal A. Mohamed
20. Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions
Emre Balbasi, Gozde Guven, and Nihal Terzi Cizmecio?lu
21. Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages
Emre Balbasi, Dersu Sezginmert, Ceren Alganatay, and Nihal Terzi Cizmecio?lu
22. Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling
Rubens Gomes-Junior, Patricia Shigunov, Bruno Dallagiovanna, and Isabela Tiemy Pereira
23. Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors
Giulia Pavani, Joshua Klein, Deborah L. French, and Paul Gadue
24. Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS
Jirina Tyleckova, Jakub Cervenka, Ievgeniia Poliakh, Jaromir Novak, Katerina Vodickova Kepkova, Helena Kupcova Skalnikova, and Petr Vodicka
