Phage Display: Methods and Protocols (Hardcover, 2018)

Preface... Table of Contents... Contributing Authors... Part I Construction of Antibody Phage Display Libraries 1. Construction of Human Immune and Naive scFv Libraries Jonas Kugler, Florian Tomszak, Andre Frenzel, and Michael Hust 2. Construction of Naive and Immune Human Fab Phage Display Library Noorsharmimi Omar and Theam Soon Lim 3. Construction of Synthetic Antibody Phage Display Libraries Johan Nilvebrant and Sachdev S. Sidhu 4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu 5. Construction of Macaque Immune Libraries Arnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat 6. Construction o f Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) Format Ulrike S. Diesterbeck 7. Construction of Rabbit Immune Antibody Libraries Thi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim 8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries Julius Grzeschik, Doreen Konning, Simon Krah, Christian Schroter, Martin Empting, Harald Kolmar, and Stefan Zielonka 9. Construction of High-Quality Camel Immune Antibody Libraries Ema Romao, Vianney Poignavent, Cecile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion 10. Construction of Chicken Antibody Libraries Jeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt 11. Construction and Selection of Affilin Phage Display Libraries Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva Bosse-Doenecke 12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies Deborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau 13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung Part II Selection Strategies for Antibodies 14. Parallelized Antibody Selection in Microtiter Plates Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, Andre Frenzel, and Michael Hust 15. Mass Spectrometry Immuno Assay (MSIA (TM)) Streptavidin Disposable Automation Research Tips (D.A.R.T's®) Antibody Phage Display Biopanning Chai Fung Chin, Yee Siew Choong, and Theam Son Lim 16. Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection Angela Chiew Wen Ch'ng, Nurul Hamizah binti Hamidon, Zoltan Konthur, and Thean Soon Lim 17. Phage Display and Selections on Cells Wieland Fahr and Andre Frenzel 18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu 19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies Almog Bitton, Limor Nahary, and Itai Benhar 20. Screening Phage Display Antibody Libraries using Protein arrays Ricardo Jara-Acevedo, Paula Diez, Maria Gonzalez-Gonzalez, Rosa Maria Degano, Nieves Ibarrola, Rafael Gongora, Alberto Orfao, and Manuel Fuentes 21. Antibody Selection on FFPE Tissue Slides Andre ten Haaf, Stefan Gattenlohner, and Mehmet Kemal Tur 22. Antibody Affinity and Stability Maturation by Error Prone PCR Tobias Unkauf, Michael Hust, and Andre Frenzel Part III Complementary Approaches for Antibody Phage Display Selections 23. Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data Christian Grohmann and Michael Blank 24. Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction Kevin A. Henry 25. High-Throughput IgG Reformatting and Expressions Chao-Guang Chen, Georgina Sansome. Michael J. Wilson, and Con Panousis