Phage Display: Methods and Protocols (Paperback, Softcover Repri)

Preface…

Table of Contents…

Contributing Authors…

Part I Construction of Antibody Phage Display Libraries

1. Construction of Human Immune and Naive scFv Libraries

Jonas Kugler, Florian Tomszak, Andre Frenzel, and Michael Hust

2. Construction of Naive and Immune Human Fab Phage Display Library

Noorsharmimi Omar and Theam Soon Lim

3. Construction of Synthetic Antibody Phage Display Libraries

Johan Nilvebrant and Sachdev S. Sidhu

4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene Cassettes

Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu

5. Construction of Macaque Immune Libraries

Arnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat

6. Construction o

Ulrike S. Diesterbeck

7. Construction of Rabbit Immune Antibody Libraries

Thi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim

8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody Libraries

Julius Grzeschik, Doreen Konning, Simon Krah, Christian Schroter, Martin Empting, Harald Kolmar, and Stefan Zielonka

9. Construction of High-Quality Camel Immune Antibody Libraries

Ema Romao, Vianney Poignavent, Cecile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion

10. Construction of Chicken Antibody Libraries

Jeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt

11. Construction and Selection of Affilin Phage Display Libraries

Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva

12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies

Deborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau

13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach

Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung

Part II Selection Strategies for Antibodies

14. Parallelized Antibody Selection in Microtiter Plates

Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, Andre Frenzel, and Michael Hust

15. Mass Spectrometry Immuno Assay (MSIA ™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning

Chai Fung Chin, Yee Siew Choong, and Theam Son Lim

16. Magnetic Nanoparticle-Based Semi-Automated Panning for

Angela Chiew Wen Ch’ng, Nurul Hamizah binti Hamidon, Zoltan Konthur, and Thean Soon Lim

17. Phage Display and Selections on Cells

Wieland Fahr and Andre Frenzel

18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens

Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu

19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain Antibodies

Almog Bitton, Limor Nahary, and Itai Benhar

20. Screening Phage Display Antibody Libraries using Protein arrays

Ricardo Jara-Acevedo, Paula Diez, Maria Gonzalez-Gonzalez, Rosa Maria Degano, Nieves Ibarrola, Rafael Gongora, Alberto Orfao, and Manuel Fuentes

21. Antibody

Andre ten Haaf, Stefan Gattenlohner, and Mehmet Kemal Tur

22. Antibody Affinity and Stability Maturation by Error Prone PCR

Tobias Unkauf, Michael Hust, and Andre Frenzel

Part III Complementary Approaches for Antibody Phage Display Selections

23. Upgrading Affinity Screening Experiments by Analysis of Next Generation Sequencing Data?

Christian Grohmann and Michael Blank

24. Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Library Construction

Kevin A. Henry

25. High-Throughput IgG Reformatting and Expressions

Chao-Guang Chen, Georgina Sansome. Michael J. Wilson, and Con Panousis

26. Monitoring Phage Biopanning by Next-Generation Sequencing

Anna Vaisman-Mentesh and Yariv Wine

Part IV Phage Display for Epitope Mapping and Identification of Biomarkers

27. ORFeome Phage Display

Jonas Zantow, Gustavo Marcal Schmidt Garcia Moreira, Stefan Dubel, and Michael Hust

28. Epitope Mapping by Phage Display

Gustavo Marcal Schmidt Garcia Moreira, Viola Fuhner, Michael Hust

29. Metasecretome Phage Display

Milica Ciric, Filomena Ng, Jasna Rakonjac, and Dragana Gagic

30. Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages

Gertrudis Rojas and Tania Carmenate

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