Sertoli Cells: Methods and Protocols (Paperback, Softcover Repri)

1. Establishment of Primary Culture of Sertoli Cells Raquel L. Bernardino, Marco G. Alves, and Pedro F. Oliveira 2. Evaluation of the Purity of Sertoli Cell Primary Cultures Raquel L. Bernardino, Marco G. Alves, and Pedro F. Oliveira 3. Preparation of Testicular Samples for Histology and Immunohistochemistry Barbara Bilinska, Anna Hejmej, and Malgorzata Kotula-Balak 4. Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age Valeria Grieco and Barbara Banco 5. Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy Jesus Martinez-Hernandez, Vicente Seco-Rovira, Ester Beltran-Frutos, Victor Quesada-Cubo, Concepcion Ferrer, and Luis Miguel Pastor 6. Sertoli Cells Preparation for Co-Immunoprecipitation Maria Joao Freitas and Margarida Fardilha 7. Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation Maria Joao Freitas and Margarida Fardilha 8. Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy Marina G. Yefimova, Nadia Messaddeq, Annie-Claire Meunier, Anne Cantereau, Bernard Jegou, and Nicolas Bourmeyster 9. A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, and Yoichi Kondo 10. Assessing Autophagy in Sertoli Cells Chao Liu, Jehangir Khan, and Wei Li 11. Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells Luis Crisostomo, Marco G. Alves, Agostina Gorga, Mario Sousa, Maria F. Riera, Maria N. Galardo, Silvina B. Meroni, and Pedro F. Oliveira 12. Assessing Sertoli Cells Metabolic Activity Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui Carvalho, Maria Noel Galardo, Maria Fernanda Riera, Silvina Meroni, and Marco G. Alves 13. Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy Rita Ferreira, Fabio Trindade, and Rui Vitorino 14. Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Weihui Zhang, and Zuping He 15. Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice Diane Rebourcet, Annalucia Darbey, Michael Curley, Peter O'Shaughnessy, and Lee B. Smith 16. Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons Qing Wen, Elizabeth I. Tang, Nan Li, Dolores D. Mruk, Will M. Lee, Bruno Silvestrini, and C. Yan Cheng 17. Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay Haiqi Chen, Wing-yee Lui, Dolores Mruk, Xiang Xiao, Renshan Ge, Qingquan Lian, Will M. Lee, Bruno Silvestrini, and C. Yan Cheng 18. Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell Pranitha Jenardhanan, Manivel Panneerselvam, and Premendu P. Mathur 19. A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells Anna Maggio, Raquel L. Bernardino, Patrizia Gena, Marco G. Alves, Pedro F. Oliveira, and Giuseppe Calamita 20. Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing Yoni Baert, Jaime Onofre, Dorien Van Saen, and Ellen Goossens