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· 분류 : 외국도서 > 과학/수학/생태 > 과학 > 생명과학 > 유전학/유전체학
· ISBN : 9781493995226
· 쪽수 : 336쪽
· 출판일 : 2020-08-14
목차
Part I: Depletion Systems to Assess SMC Function
1. Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo
Demis Menolfi and Dana Branzei
2. Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System
Clementine Brocas, Cecile Ducrot, and Karine Dubrana
3. Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation
Yasutaka Kakui and Frank Uhlmann
4. Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts
Himaja Gaddipati, Marina V. Pryzhkova, and Philip W. Jordan
Part II: Genetic Manipulation of SMC Function
5. High-Throughput Allelic Replacement Screening in Bacillus subtilis
Marie-Laure Diebold-Durand, Frank Burmann, and Stephan Gruber
6. Identifying Functional Domains in Subunits of Structural Maintenance of Chromosome (SMC) Complexes by Transposon Mutagenesis Screen in Yeast
Avi Matityahu, Michal Shwartz, and Itay Onn
7. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes
Jan Josef Pale?ek, Lucie Vondrova, Kate?ina Zabrady, and Jakub Oto?ka
8. Knocking in Multi-Functional Gene Tags into SMC Complex Subunits Using Gene Editing
Paul Kalitsis, Tao Zhang, Ji Hun Kim, Christian F. Nielsen, Kathryn M. Marshall, and Damien F. Hudson
Part III: Chromosomal Assays of SMC Activity
9. Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenance of Chromosomes Complex In Vivo
Tung B.K. Le
10. Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation
Vasso Makrantoni, Daniel Robertson, and Adele L. Marston
11. Analysis of Cohesin Association to Newly Replicated DNA through Nascent Strand Binding Assay (NSBA)
Camilla Frattini and Rodrigo Bermejo
12. Preparation of Cell Cycle Synchronized Saccharomyces cerevisiae Cells for Hi-C
Stephanie A. Schalbetter and Jonathan Baxter
Part IV: Biochemical Assays of SMC Activity
13. Dissecting DNA Compaction by the Bacterial Condensin MukB
Rupesh Kumar, Soon Bahng, and Kenneth J. Marians
14. In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA
Koichi Yano, Koichiro Akiyama, and Hironori Niki
15. A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexes
Menelaos Voulgaris and Thomas G. Gligoris
16. In Vitro Detection of Long Noncoding RNA Generated from DNA Double-Strand Breaks
Sheetal Sharma and Fabrizio d’Adda di Fagagna
Part V: Microscopy-Based Assays of SMC Activity
17. Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging
Suchitha Raghunathan and Anjana Badrinarayanan
18. Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus under DNA Damage
Afroze Chimthanawala and Anjana Badrinarayanan
19. Microinjection Techniques in Fly Embryos to Study the Function and Dynamics of SMC Complexes
Catarina Carmo, Margarida Araujo, and Raquel A. Oliveira
20. Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex
Logan R. Myler, Michael M. Soniat, Xiaoming Zhang, Rajashree A. Deshpande, Tanya T. Paull, and Ilya J. Finkelstein
Part VI: Theoretical Modeling of SMC Activity
21. Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase
Josh Lawrimore, Yunyan He, M. Gregory Forest, and Kerry Bloom
22. Molecular Dynamics Simulations of Condensin-Mediated Mitotic Chromosome Assembly
Yuji Sakai, Tatsuya Hirano, and Masashi Tachikawa
