Plant Virology: Methods and Protocols (Paperback, 2022)

Preface…
Table of Contents…
Contributing Authors…

1 In Vivo Detection of Double-Stranded RNA by dRBFC Assay
Xiaofei Cheng, Yameng Luan, and Aiming Wang

2 Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing Technology
Keyan Xu and Yi Li

3 Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions
Yu Huang and Yi Li

4 Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors 
Fangfang Li and Aiming Wang

5 Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research 
Zhaoji Dai and Aiming Wang

6 Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions
Yan Liang and Zhenghe Li

7 Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants 
Zhaoji Dai and Aiming Wang 

8 Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay 
Ziwei Tang, Mark A. Bernards, and Aiming Wang

9 A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay 
Zhihao Jiang, Meng Yang, Qing Cao, Yongliang Zhang, and Dawei Li

10 Membrane and Nuclear Yeast Two-Hybrid Systems
Qian Chen and Taiyun Wei

11 Tag-Based Pull-Down Assay 
Shanwu Lyu, Changwei Zhang, Xilin Hou, and Aiming Wang

12 Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses
Rongrong He, Mark A. Bernards, and Aiming Wang 

13 Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants
Aaron Simkowich, Susanne E. Kohalmi, and Aiming Wang 

14 Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity 
Shanshan Zhao and Jianguo Wu

15 Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors 
Tao Zhou, Laihua Dong, Tong Jiang, and Zaifeng Fan 

16 A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus
Ji-Hui Qiao, Qiang Gao, Ying Zang, Xiao-Dong Fang, and Xian-Bing Wang 

17 Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques
Keru Yan, Xuejie Du, and Bizeng Mao

18 Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips
Min-Rui Wang, Tianxing Pang, Ziqian Lian, Qiao-Chun Wang, and Liying Sun 

19 Establishment of White-Backed Planthopper Cell Lines 
Hongyan Chen, Wei Wu, and Taiyun Wei

20 Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses 
Yinzi Li and Aiming Wang

21 Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues
Yuxin Ma and Shifang Li

22 Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses
Ali Raza and Qingfa Wu

23 Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses
Subhas Hajeri and Raymond Yokomi

24 Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA 
Shibo Gao and Jianxiang Wu 

25 Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees 
Liping Wang

26 Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR
Yi Xie and Jianxiang Wu

27 RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants 
Jing-Ze Zou, De-Shui Liu, Xin Tong, Xiao-Peng Zhang, and Xian-Bing Wang

28 Transmission Electron Microscopic Methods for Plant Virology 
Li Xie and Jian Hong 

Subject Index List…