Fluorescent Protein-Based Biosensors: Methods and Protocols (Paperback, Softcover Repri)

1. The Design and Application of Genetically-Encodable Biosensors Based on Fluorescent Proteins Robert H. Newman and Jin Zhang 2. An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications J. Goedhart, Mark A. Hink, and Kees Jalink 3. Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells using Genetically Encoded Fluorescent Biosensors J. Genevieve Park and Amy E. Palmer 4. Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells J. Klarenbeek and Kees Jalink 5. Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters Alessandra Stangherlin, Andreas Koschinski, Anna Terrin, Anna Zoccarato, He Jiang, Laura Ashley Fields, and Manuela Zaccolo 6. Genetically Encoded Fluorescent Biosensors for Live-cell Imaging of Lipid Dynamics Moritoshi Sato 7. Live Cell Imaging of Cytosolic NADH-NAD+ Redox State using a Genetically Encoded Fluorescent Biosensor Yin Pun Hung and Gary Yellen 8. Measuring Membrane Voltage with Microbial Rhodopsins Daniel R. Hochbaum and Adam Cohen 9. Imaging the Activity of Ras superfamily GTPase Proteins in Small Subcellular Compartments in Neurons Ana F. Oliveira and Ryohei Yasuda 10. Imaging Kinase Activity at Protein Scaffolds Maya T. Kunkel and Alexandra C. Newton 11. Using a Genetically Encoded FRET-Based Reporter to Visualize calcineurin phosphatase Activity in Living Cells Sohum Meta and Jin Zhang 12. Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation Kazuki Sasaki and Minoru Yoshida 13. Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang 14. Biosensor Imaging in Brain Slice Preparations Marina Polito, Pierre Vincent, and Elvire Guiot 15. Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster Shubham Dipt, Thomas Riemensperger, and Andre Fiala 16. Multi-Parameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel Nwe-Nwe Aye-Han and Jin Zhang 17. FRET and BRET-Based Biosensors in Live Cell Compound Screens Katie Herbst-Robinson, Jessica Yang, and Jin Zhang 18. Integrating Fluorescent Biosensor Data Using Computational Models Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffery J. Saucerman