Intrinsically Disordered Protein Analysis: Volume 1, Methods and Experimental Tools (Paperback, Softcover Repri)

Part I. Assessing IDPs in the Living Cell 1. Determination of IUP Based on Susceptibility for Degradation by Default Peter Tsvetkov and Yosef Shaul 2. In-cell NMR of Intrinsically Disordered Proteins. Prokaryotic Cells Yutaka Ito, Tsutomu Mikawa and Brian O. Smith 3. In-cell NMR in Xenopus laevis Oocytes Rossukon Thongwichian and Philipp Selenko 4. In-cell NMR in Mammalian Cells: Part 1 Beata Bekei, Honor May Rose, Michaela Herzig, Alexander Dose, Dirk Schwarzer, and Philipp Selenko 5. In-cell NMR in Mammalian Cells: Part 2 Beata Bekei, Honor May Rose, Michaela Herzig and Philipp Selenko 6. In-cell NMR in Mammalian Cells: Part 3 Beata Bekei, Honor May Rose, Michaela Herzig, Heike Stephanowitz, Eberhard Krause, and Philipp Selenko 7. Fourier Transform Infrared Microspectroscopy of Complex Biological Systems: from Intact Cells to Whole Organisms Diletta Ami, Antonino Natalello, and Silvia Maria Doglia 8. Studying IDP Stability and Dynamics by Fast Relaxation Imaging in Living Cells Apratim Dhar, Maxim Prigozhin, Hannah Gelman, and Martin Gruebele Part II. NMR-Based Techniques 9. Measurement and Analysis of NMR Residual Dipolar Couplings for the Study of Intrinsically Disordered Proteins Loic Salmon, Malene Ringkjøbing Jensen, Pau Bernado, Martin Blackledge 10. Distance Information for Disordered Proteins from NMR and ESR Measurements using Paramagnetic Spin Labels David Eliezer 11. Using Chemical Shifts to Assess Transient Secondary Structure and Generate Ensemble Structures of Intrinsically Disordered Proteins Stepan Kashtanov, Wade Borcherds, Hongwei Wu, Gary W. Daughdrill, F. Marty Ytreberg 12. Magic Angle Spinning Solid State NMR Experiments for Structural Characterization of Proteins Lichi Shi and Vladimir Ladizhansky 13. Wide-line NMR and Protein Hydration Tompa K. Bokor M. Tompa P. 14. 5-Fluorotryptophan as a Dual NMR and Fluorescent Probe of a-Synuclein Candace M. Pfefferkorn and Jennifer C. Lee 15. Aplpha Proton Detection Based on Backbone Assignment of Intrinsically Disordered Proteins Part III. Vibrational Spectroscopy 16. Fourier Transform Infrared Spectroscopy of Intrinsically Disordered Proteins: Measurement Procedures and Data Analyses Antonino Natalello, Diletta Ami, and Silvia Maria Doglia 17. Monitoring Stuctural Transitions in IDPs by Vibrational Spectroscopy of Cyanlated Cysteine Hailiu Yang, Johnny Habchi, Sonia Longhi, Casey H. Londergan 18. Structure Analysis of Unfolded Peptides by Vibrational Circular Dichroism Spectroscopy Reinhard Schweitzer-Stenner, Jonathan B. Soffer and Daniel Verbaro 19. Structural Analysis of Unfolded Peptides by Raman Spectroscopy Reinhard Schweitzer-Stenner, Jonathan B. Soffer, Siobhan Toal and Daniel Verbaro 20. Isotope-Edited Infrared Spectroscopy Ginka S. Buchner and Jan Kubelka Part IV. Other Spectroscopic Techniques 21. Monitoring Structural Transitions in IDPs by site-directed Spinlabeling EPR Spectroscopy Johnny Habchi, Marlene Martinho, Antoine Gruet, Bruno Guigliarelli, Sonia Longhi and Valerie Belle 22. Circular Dichroism Techniques for the Analysis of Intrinsically Disordered Proteins and Domains Lucia B. Chemes, Leonardo G. Alonso, Maria G. Noval and Gonzalo de Prat-Gay 23. Deconstructing Time-resolved Optical Rotatory Dispersion Kinetic Measurements of Cytochrome c Folding: From Molten Globule to the Native State Eefei Chen and David S. Kliger 24. The use of UV-VIS Absorption Spectroscopy for Analysis of Natively Disordered Proteins Eugene A. Permyakov 25. Intrinsic Fluorescence of Intrinsically Disordered Proteins Paolo Neyroz and Stefano Ciurli 26. Binding Stoichiometry and Affinity of Fluorescent Dyes to Proteins in Different Structural States Anna I. Sulatskaya, Olga I. Povarova, Irina M. Kuznetsova, Vladimir Uversky and Konstantin K. Turoverov 27. Fluoresnece Lifetime Measurements of I