Metabolic Profiling: Methods and Protocols (Hardcover, 2018)

Preface... Table of Contents... Contributing Authors... Part I Fundamentals 1. Metabolic Profiling: Topic, Challenges, Perspective Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson 2. Quality Control and Validation Issues in LC-MS Metabolomics Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson 3. Data Treatment for LC-MS Untargeted Analysis Samantha Riccadonna and Pietro Franceschi 4. Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis Michael Witting Part II Methods 5. HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis 6. Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition Filippos Michopoulos 7. LC-MS Untargeted Analysis Elizabeth Want 8. NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts Dimitra Benaki and Emmanuel Mikros 9. Untargeted GC-MS Metabolomics atthaios- mmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa 10. Rat Fecal Metabolomics Based Analysis Olga Deda, Helen G. Gika, and Georgios A. Theodoridis 11. GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol Petr Husek, Zdenek Svagera, Dagmar Hanzlikova, Iva Opekarova, Lucie Rimnacova, Helena Zahradnickova, and Petr Simek 12. Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples Rawi Ramautar Part III Plant/Food and Other Applications 13. Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR Jan Schripsema and Denise Dagnino 14. NMR Spectroscopy Protocols for Food Metabolomics Applications Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros 15. Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study Brian Farneti 16. LC-MS Untargeted Protocol for the Analysis of Wine Panagiotis Arapitsas and Fulvio Mattivi Part IV Life Science Applications 17. Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li 18. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics) Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, and Serge Rudaz 19. Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation Aikaterina Siopi and Vassilis Mougios