Plant Functional Genomics: Methods and Protocols (Hardcover, 2003)

"Part I. Finding Genes in Complex Plant Systems An Improved Method for Plant BAC Library Construction Meizhong Luo and Rod A. Wing Constructing Gene-Enriched Plant Genomic Libraries Using Methylation Filtration Technology Pablo D. Rabinowicz RescueMu Protocols for Maize Functional Genomics Manish N. Raizada Precious Cells Contain Precious Information: Strategies and Pitfalls in Expression Analysis from a Few Cells Isabelle M. Henry and Dina F. Mandoli Combined ESTs from Plant-Microbe Interactions: Using GC Counting to Determine the Species of Origin Edgar Huitema, Trudy A. Torto, Allison Styer, and Sophien Kamoun Part II. In Silico Prediction of Plant Gene Function Computer Software to Find Genes in Plant Genomic DNA Ramana V. Davuluri and Michael Q. Zhang Genomic Colinearity as a Tool for Plant Gene Isolation Wusirika Ramakrishna and Jeffrey L. Bennetzen Using Natural Allelic Diversity to Evaluate Gene Function Sherry R. Whitt and Edward S. Buckler, IV Quantitative Trait Locus Analysis as a Gene Discovery Tool Michael D. McMullen Part III. Forward and Reverse Genetic Strategies Transposon Tagging Using Activator (Ac) in Maize Thomas P. Brutnell and Liza J. Conrad T-DNA Mutagenesis in Arabidopsis Jose M. Alonso and Anna N. Stepanova Physical and Chemical Mutagenesis Andrea Kodym and Rownak Afza High-Throughput TILLING for Functional Genomics Bradley J. Till, Trenton Colbert, Rachel Tompa, Linda C. Enns, Christine A. Codomo, Jessica E. Johnson, Steven H. Reynolds, Jorja G. Henikoff, Elizabeth A. Greene, Michael N. Steine, Luca Comai, and Steven Henikoff Gene and Enhancer Traps for Gene Discovery Marcela Rojas-Pierce and Patricia S. Springer High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Inserti