Redox-Mediated Signal Transduction: Methods and Protocols (Hardcover, 2009)

Chapter 1: The role of redox in signal transduction John T. Hancock Chapter 2: The measurement of nitric oxide and its metabolites in biological samples by ozone-based chemiluminescence. Andrew G. Pinder, Stephen C. Rogers, Afshin Khalatbari, Thomas E. Ingram, and Philip E. James. Chapter 3: Detection and measurement of reactive oxygen intermediates in mitochondria and cells Matthew Whiteman, Yuktee Dogra, Paul G. Winyard and Jeffrey S. Armstrong Chapter 4: Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Review Mark B. Cannon and S. James Remington Chapter 5: Measuring redox changes in vivo in leaves: prospects and technical challenges Philip M. Mullineaux and Tracy Lawson Chapter 6: Imaging of intracellular hydrogen peroxide production with HyPer upon stimulation of HeLa cells with EGF Kseniya N. Markvicheva, Ekaterina A. Bogdanova, Dmitry B. Staroverov, Sergei Lukyanov, Vsevolod V. Belousov Chapter 7: Tools to investigate ROS sensitive signalling proteins Radhika Desikan, Steven J. Neill, Jenna Slinn and John T. Hancock Chapter 8: Methods for preparing crystals of reversibly oxidized proteins: crystallization of protein tyrosine phosphatase 1B as an example. Annette Salmeen and David Barford Chapter 9: Methods for the Study of Redox-Mediated Changes in p53 Structure and Function Kristine Mann Chapter 10: Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase. Susan M. Hutson, Leslie B. Poole, Steven Coles and Myra E. Conway Chapter 11: Detection of carbonylated proteins in 2-D SDS PAGE separations. Rukhsana Sultana, Shelley F. Newman, Quanzhen Huang, D. Allan Butterfield Chapter 12: Analysis of global and specific changes in the disulfide proteome using Redox 2D-PAGE Robert C. Cumming Chapter 13: Protein-thiol oxidation, from single proteins to proteome-wide analyses Natacha Le Moan, Frederique Tacnet and Michel B. Toledano Chapter 14: Analysis of redox relationships in the plant cell cycle: determinations of ascorbate, glutathione and poly (ADPribose)polymerase (PARP) in plant cell cultures Christine H. Foyer, Till K. Pellny, Vittoria Locato and Laura De Gara Chapter 15: Generation and Detection of S-Nitrosothiols Christian Lindermayr, Simone Sell, and Jorg Durner