The Protein Protocols Handbook (Paperback, 3)

Part I : QUANTITATION OF PROTEINS 1. Protein Determination by UV Absorption A. Aitken and M.P. Learmonth 2. The Lowry Method for Protein Quantitation J. H. Waterborg 3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation J.M. Walker 4 The Bradford Method for Protein Quantitation N.Kruger 5. Ultrafast Protein Determinations Using Microwave Enhancement R. Akins and R. S. Tuan 6. The Nitric Acid Methods for Protein Estimation in Biological Samples S.A. Boerner, Y.K. Lee, S.H. Kaufmann and K. Bible 7. Quantitation of Tryptophan in Proteins A. Aitken and M.P. Learmonth 8. Kinetic Silver Staining of Proteins D. D. Root and K. Wang 9. Quantitation of cellular proteins by flow cytometry T. D. Friedrich , F.A. Ray, R.L. Smith and J. M. Lehman 10. Quantitation of cellular proteins by laser scanning cytometry T.D. Friedrich, R.L.Smith and J.M. Lehman Part II : ELECTROPHORESIS OF PROTEINS AND PEPTIDES AND DETECTION IN GELS 11 Protein Solubility in 2D Electrophoresis: Basic principles and issues T.Rabilloud 12 Mouse and Human Tissues Sample Preparation for 2-D Electrophoresis C. Zabel and J. Klose 13 Plant Protein sample preparation for 2DE S. Carpentier , R.Swennen and B. Panis 14 Preparation of bacterial samples for 2-D PAGE. B. Vandahl, G. Christiansen and S. Birkelund 15 Preparation of bodily fluids for 2-D PAGE Sullivan, H.Brzeski, J. Ganesalingam, and M. Mayr 16 Immunoaffinity Depletion of high abundance plasma and serum proteins L.A. Echan and D. Speicher 17 Preparation of Yeast samples for 2D PAGE J. Norbeck 18 Membrane Protein Preparation Using Aqueous Polymer Two Phase Systems J. Schindler and H-G Nothwang 19 Subcellular fractionation of small sample amounts H.G Nothwang, I. Guillemin and J. Schindler 20. Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins J.M. Walker 21 SDS Polyacrylamide Gel Electrophoresis of Proteins J.M. Walker 22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins J.M. Walker 23. SDS-Polyacrylamide Gel Electrophoresis of Peptides R. C. Judd 24 Blue native gel electrophoresis (BN-PAGE) Elke A. Dian, J. Rassow and Christian Motz 25 Separation of proteins by gel electrophoresis in the Tris-Taurine-HCl system S.Luche, M. Chevallet C. Lelong and T. Rabilloud 26 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M -Based Separation of Proteins with Retained Native Activity R.E. Akins and R. S. Tuan 27 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins J. H. Waterborg 28 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones J. H. Waterborg 29 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels J. M. Walker 30 Serial immobilized pH gradient isoelectric focusing over pH 4-9 S.Poznanovic, W. Wozny, H. Zengerling, G.P. Schwall and M. A. Cahill 31. Radiolabelling of Eukaryotic Cells and Subsequent Preparation for 2-D 43 Quantitation of Proteins on Polyacrylamide Gels J. Smith 44 Using SDS-PAGE and scanning laser densitometry to measure proteins Aaron P. Miles and A. Saul 45 Rapid and Sensitive Staining of Unfixed Proteins in Polyacrylamide Gels with Nile Red J-R. Daban, S. B.A. Bermudez amd F.J. Alba 46 Zinc reverse staining technique C. Fernandez-Patron 47 Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels Wei-Tao Cong, Sun-Young Hwang, Li-Tai Jin and Jung-Kap Choi 48 Detection of Proteins in Polyacrylamide Gels by Silver Staining M. J. Dunn 49 Background-free Protein Detection on Polyacrylamide Gels and on Electroblots Using Transition Metal Chelate Stains W.F. Patton 50 Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining M. J. Dunn 51 Detection of Glycoproteins in Gels and Blots Nicolle Packer, Malcolm S Ball, Peter L Devine, and Wayne F Patton 52 Stai