Proteomics in Functional Genomics: Protein Structure Analysis (Hardcover, 2000)

Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis.- Techniques for sample preparation including methods for concentrating peptide samples.- Sample preparation by SDS/PAGE and in-gel digestion.- High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection.- Nanospray mass spectrometry in protein and peptide chemistry.- MALDI-TOF mass spectrometry in protein chemistry.- The chemistry of protein sequence analysis.- The alkylated thiohydantoin method for C-terminal sequence analysis.- Ladder Sequencing.- New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer.- Analysis of glycoprotein-derived glycopeptides.- Lipopeptide preparation and analysis.- Phosphopeptide analysis.- Bioinformatics in protein analysis.