분자생물학 그림여행 CLASS

Part 1. DNA의 구조와 기능

1. Nucleotides
1.1 Base
1.2 Sugar
1.3 Phosphate
1.4 Bonds
1.4.1 Glycosidic Bond
1.4.2 Phosphodiester Bond
1.4.3 Hydrogen Bond (수소결합)
1.4.4 Base Stacking

* 시험문제

2. Helices
2.1 Watson-Crick Helix (B form)
2.2 A Form Helix
2.3 Z Form Helix
2.4 Denaturation과 Renaturation
2.4.1 Melting Curve
2.4.2 Hysteresis
2.4.3 High pH에서의 Denatuaration
2.4.4 Organic Solvent에 의한 Denaturation

* 시험문제

3. Tertiary Structure of DNA
3.1 Circular DNA에서의 Supertwist
3.1.1 Supertwisted DNA의 발견
3.1.2 Topological Bond
3.1.3 Sings of Supertwist
3.1.4 두 가지 모양의 Supertwist
3.1.5 Linking Number (LK)
3.1.6 Superhelical Density의 측정
3.2 Linear DNA에서의 Supertwist
3.3 Supertwisted DNA의 생물학적 의의
3.4 Supertwisted Density의 Control

* 시험문제

4. Choromatin Structure
4.1 Nucleosome
4.2 Solenoidal Fibre (30 nm chromatin fibre)
4.3 Loop Structure

* 시험문제

5장 Gradient Centrifugation의 원리와 응용
5.1 Types of Gradient
5.1.1 Valocity Type
5.1.2 Equilibrium Type
5.2 여섯종류의 Gradient
5.2.1 High Salt Neutral Sucrose Velocity Gradient
5.2.2 Low Salt Neutral Sucrose Velocity Gradient
5.2.3 Alkaline Sucrose Velocity Gradient
5.2.4 Neutral CsCl Equilibrium Density Gradient
5.2.5 Alkaline CsCl Equilibrium Density Gradient
5.2.6 Neutral CsCl-ErBr Equilibrium Density Gradient

* 시험문제

Part 2. DNA와 만나는 효소들의 생명현상 운영

1. DNA Polymerase
1.1 DNA Polymerase I
1.2 T4 DNA Polymerase
1.3 E. coli DNA Polymerase II
1.4 Eucaryotic DNA Polymerase

2. Nuclease
* 시험문제

3장 DNA Ligase
3.1 DNA Ligase의 발견
3.2 DNA Ligase의 특성

4. Single Strand DNA Binding Protein (ssb)
4.1 Discovery
4.2 ssb의 특성
4.3 여러 종류의 Single Strand DNA Binding Protein
4.4 ss DNA와 ds DNA의 Transition

* 시험문제

5. Topoisomerase
5.1 Type I Topoisomerase
5.2 Type II Topoisomerase
5.3 Maintaining Superhelical Density
5.4 Classification
5.5 Topoisomerase의 in vivo Function

* 시험문제

Part 3. DNA 복제의 기본원칙과 연구과정

1. In vivo DNA Synthesis
1.1 Semiconservative DNA Replication
1.2 Replicon Model
1.3 DNA Dilemma
1.4 DNA Neodilemma
1.5 Completion of Replication
1.6 Origin에서의 Priming

2. E. coli DNA Replication
2.1 Mustant Study
2.2 Single Strand DNA Phage Replication
2.3 T7 Replication

3. Initiation of DNA Replication
3.1 E. coli Replication Origin
3.2 3가지의 Repeat 구조
3.3 Initiation Mechanism

4. DNA 복제 조절
4.1 ColE1 Plasmid Replication

5. SV40 DNA Replication

6. Eucaryotic DNA Replication

* 시험문제